摘要
目的：建立一种简单实用的胶体金免疫层析法用于食品样品中大肠杆菌O157：H7的快速筛查。方法：采用柠檬酸三纳还原法制备胶体金颗粒．标记抗E．coli0157：H7单克隆抗体．制备免疫胶体金复合物，组装胶体金免疫层析快速检测试纸条，并对其特异性、灵敏度和稳定性进行了测试和评价。结果：该试纸条特异性较好．不与其他受试菌株发生交叉反应，检测灵敏度为10^5CFU／mL。120份人工污染E．coliO157：H7模拟食品样本增菌后检测，该法检测阳性118份，灵敏度高达98．3％。结论：本实验成功研制了E．coliO157：H7胶体金免疫层析试纸条，该试纸条具有灵敏度高、特异性好、检测速度快、操作简便等优点，可用于现场大量样品的初筛。
Objective To establish a simple, practical gold immunochromatography assay （GICA） for rapidly screening E.coli O157：H7 in food samples. Methods Trisodium citrate reduction was used to prepare colloidal gold pellets, which were coupled with monoclonal anli-E.coli O157：H7 to produce immune complex and assemble a GICA strip tool. The sensitivity and specificity of this strip was then evaluated. Results The GICA strip exhibited a higher specificity for detecting E.coli O157：H7 and did not cross-react with other strains. The sensitivity of the strip was l0s cfu/ml of pure bacterial culture. 118 of 120 samples artificially contaminated with E.coli O157：H7 were detected positive, with a sensitivity of up to 98.3%. Conclusions A GICA strip for detecing E.coli O157：H7, which has a higher specificity and a better specificity, is successfully produced. It is a rapid, simple method for detection and can be used for primarily screening food samples on site.
出处
《实用医学杂志》
CAS
北大核心
2010年第13期2280-2283,共4页
The Journal of Practical Medicine
基金
广东省科技计划项目(编号:2006B14701001)
