摘要
目的:观察葛根素对脂多糖诱导的急性肺损伤大鼠肺组织水通道蛋白-1(AQP1)表达、病理形态学、湿干比等的影响,探讨其对急性肺损伤的保护作用。方法:健康Wistar大鼠36只,采用腹腔内注射脂多糖(lipopolysaccharide,LPS)法复制急性肺损伤动物模型。将大鼠随机分为盐酸对照组(对照组)、LPS损伤组(损伤组)和葛根素+LPS组(葛根素组)。结果:光镜下见对照组肺泡结构清晰,肺泡腔及支气管腔未见明显炎细胞及渗出物。LPS组镜下可见肺组织水肿,表面可见暗红色点、片状出血,大量炎性细胞浸润,肺泡间隔明显增厚,葛根素+LPS组损伤较LPS组明显减轻。LPS组湿干比较对照组增高,葛根素组湿干比较LPS组降低。LPS组AQP1蛋白表达较对照组减少,葛根素组肺组织AQP1蛋白表达较LPS组明显增加。结论:葛根素对脂多糖所致的大鼠急性肺损伤具有保护作用。
Objective：The aim of present study was to observe the expression of aquaporin-1 in rats with acute lung injury induced by LPS,and discuss the protection of the puerarin in acute lung injury.Methods：A total of 36 Wistar healthy rats were randomly assigned into 3 groups：the comparison group,the injury group and the puerarin group.Result：The rat of comparison group structure of pulmonary alveolus was clear,the pulmonary alveolus wall was thin,the pulmonary alveolus had not seen fluid;the rat of injury group was unable to debate the pulmonary alveolus structure,in the lung the massive phlogocytes infiltrate（by neutral granular cell primarily） with part bleeding,the dropsy,the transparent membrane formated;The rat of the puerarin group,the pulmonary alveolus gap accumulated,the neutral granular cell infiltration obviously reduced,the pulmonary alveolus cavity saw few exudation.The expression of AQP1 protein in the injury lower than in the comparison group,puerarin group was significantly higher than in the injury group.Conclusion：Puerarin has the protective effects on ALI which resulted by the LPS in rats.
出处
《现代生物医学进展》
CAS
2010年第12期2253-2255,共3页
Progress in Modern Biomedicine
