摘要
目的:鉴定不同产地火麻仁,并探讨火麻仁的遗传多样性。方法:利用SDS-聚丙烯酰胺凝胶电泳技术,对不同外观性状的火麻仁进行蛋白电泳聚类分析。结果:火麻仁蛋白电泳图谱表现出可观察到的谱带18条,其中7条较明显的谱带为共有谱带,另外11条谱带具有多态性,反映了火麻仁的遗传多样性。结论:火麻仁蛋白稳定且具有丰富的遗传多样性,实验结果可为准确鉴定药用火麻仁提供辅助依据。
Objective： To Identify the species and affinities of cannabis semen from different habitats, and investigate the relationships between heredity diversity and apparent property. Methods： Apply the SDS- polyacrylamide electrophoresis technology for the first time to take protein electrophoresis cluster analysis on the cannabis semen varied from apparent property. Results： Eighteen obvious spectral bands could be observed in the cannabis semen protein electrophoretogram, among which seven obvious and shared bands manifest inter-species consistency in cannabis semen. And the other eleven bands manifest the genetic polymorphism of cannabis semen. Conclusion： Protein of cannabis semen is stable and it has heredity diversity in abundance. The experimental result can provide theoretical support for the identification of medicinal Cannabis sativa L..
出处
《中国现代中药》
CAS
2010年第7期28-30,共3页
Modern Chinese Medicine
关键词
大麻
火麻仁
SDS-聚丙烯酰胺凝胶电泳
Cannabis sativa L.
Cannabis semen
SDS-polyacrylamide electrophoresis
