摘要
目的：探讨细胞因子对ｒＩＬ２活化的脐血淋巴细胞粘附脐静脉血管内皮细胞（ＨＵＶＥＣ）的影响机理。方法：计数粘附率和ＡＰＡＡＰ法测细胞表型。结果：ｒＩＬ２活化淋巴细胞粘附ＨＵＶＥＣ的能力明显大于未刺激组，前者的粘附率是５６．５２％，后者是２４．５４％。ｒＩＬ１、２、８、ｒＴＮＦα可明显增强其粘附，而ｒＩＬ１０使粘附率明显下降至３８．１２％；ｒＩＬ１、８、ｒＴＮＦα增强ＣＤ４＋细胞的粘附，ｒＩＬ１０抑制ＣＤ４＋、ＣＤ５６＋的粘附。结论：活化淋巴细胞粘附ＨＵＶＥＣ的作用明显增强，ｒＩＬ１、２、８、１０和ｒＴＮＦα对这一过程具有重要的调节作用。
Objective:To explore the effects of different cytokine on the adherence of rIL 2 activated umbilical lymphocytes to HUVEC(human umbilical vein endothelial cells).Methods :Activated umbilical lymphocytes were laid on the surface of the cultured HUVEC monolayer. After a certain period of time,the adherence rate was determined. The phenotypes of adherent lymphocytes were studied with APAAP method.Results:rIL 2 activated lymphocytes exhibited a stronger adherence to HUVEC than that of control group.rIL 1(10 U/ml) rIL 2(1 000 U/ml ) rIL 8(1 mg/ml)and rTNF α(250 U/ml) could promote the adherence of rIL 2 activated lymphocytes,but rIL 10 decreased the adhesion rate to 38.12%.Conclusions:The adherent activity of activated lymphocytes is obviously stronger than that of unactivated ones and cytokine may play an important role in regulating lymphocyte adherence to vascular endothelium.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第3期114-116,共3页
Chinese Journal of Immunology
