摘要
利用不同浓度的趋化物质Ｎ－甲酰甲硫亮氨酰苯丙氨酸（ｆＭＬＰ）处理中性粒细胞，并采用硝基蓝四唑（ＮＢＴ）还原法检测中性粒细胞激活率，结合流室系统定量地研究了在不同切应力作用下，不同激活状态的中性粒细胞与人脐静脉内皮细胞（ＨＵＶＥＣ）粘附的关系。通过显微镜观察、计数及统计分析，结果表明：随着ｆＭＬＰ浓度的增加，中性粒细胞激活率显著增加，两者有着明显的量效关系。该实验还表明：中性粒细胞经ｆＭＬＰ处理后，与对照组一样，随着切应力增加，中性粒细胞与内皮细胞粘附率按指数曲线下降；中性粒细胞激活率的增加可以引起中性粒细胞－内皮细胞粘附显著增加，当切应力由小变大时，这种作用有减缓的趋势。以上结果提示：①体内血流产生的切应力可能是阻止中性粒细胞－内皮细胞粘附的重要因素，中性粒细胞粘附随切应力增加而减少有利于体内正常血液循环的维持；
Neutrophil was activated by chemotactic tripeptide N formyl methionyl leucyl phenylalanine(fMLP) of different concentration. The activation rate was tested by the method of nitroblue tetrazolium reduction. Using flow chamber system, we quantitatively studied the properties of neutrophil adhesion to human umbilial vein endothelial cell (HUVEC) under different shear stress. The results showed that the activation rate of neutrophil significantly increased with increase of fMLP concentration that the adhesion rate of neutrophil pretreated with fMLP decreased with the gradual increase of shear stress, such a change was also found in the control group; and that the adhesion rate of neutrophil to HUVEC increased with the increase of activation rate of neutrophil, but the adhesion rate had a tendency to decrease when shear stress increased. These indicated that shear stress induced by bloodstream might play an important role in inhibiting neutrophil endothelial cell adherence in vivo and the enhanced activation of neutrophil could increase neutrophil endothelial cell adherence.
出处
《华西医科大学学报》
CAS
CSCD
1999年第1期8-11,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
关键词
白细胞
激活
粘附
心血管病
脑血管病
HUVEC
Neutrophil Activation Adherence N formyl methionyl leucyl phenylalanine Human umbilical vein endothelial cell
