摘要
目的筛选出连通多孔钛表面的最佳生物活性处理方法。方法将高温烧结法制备出的连通多孔钛材料在5MNaOH溶液中浸泡处理24h后,然后分成三组(A、B、C组)并分别浸泡在配制好的三种浓度(1.0、1.5和2.0)的模拟体液(SBF)中。每组材料按照浸泡时间的不同又分为三个亚组(即10d、14d和18d组)。另取连通多孔钛材料直接浸泡于1.5倍SBF中(D组)并按浸泡时间不同分成10d、14d和18d三个亚组。将浸泡结束后的材料取出并经去离子水超声清洗及50℃烘干后,利用光学显微镜和扫描电镜对材料表面进行观察。结果扫描电镜对各组浸泡后的连通多孔钛材料进行表面观察结果显示,A组和C组浸泡10和14d后,所形成的类骨磷灰石均很少,都未形成完整的一层;B组浸泡7d后,表面可见散在、薄层类骨磷灰石层;14d后,其表面可见一完整的类骨磷灰石层,而浸泡18d后,其表面出现较多的空洞并有裂纹;D组浸泡10、14和18d后,扫描电镜显示其形成的类骨磷灰石均很少。结论连通多孔钛材料经过5MNaOH溶液处理并在1.5倍浓度的模拟体液中浸泡14天后可获得最佳的表面活性处理效果。
Objective To screen the optimal surface treatment of the interconnected porous titanium （IPT）. Methods After 24 h of pretreatment with 5 mol/L NaOH solution, the IPT was divided into 3 groups （A, B, and C） and immersed into three different concentrations of simulated body fluid （1.0, 1.5, and 2.0 SBF） respectively. Every group was divided into 3 subgroups according to the immerdsing time （10-, 14-, and 18-day subgroup）. In group D, IPT was immersed directly into 1.5 SBF, which was also divided into 3 subgroups according to immersing time （10-, 14-, and 18-day subgroup）. After the immersion, All these IPT materisl were washed out with deionized water in an ultrasonic washer and dried at 50 ℃. Under the light microscopy and scanning electron microscopy, the surface of the IPT material was observed. Results Very few bonelike apatite was deposited on the surface of the IPT in groups A, C and D. In group B, a thin layer of bonelike apatite on the surface of the IPT was observed in 7-day subgroup, and entire layer of bonelike apatite observed in 14-day subgroup. But in 18-day subgroup, there were many cavities and cracks in developed bonelike apatite layer. Conclusion The optimum bioactive treatment of the IPT material was as follows： immersion of the IPT into 1.5 SBF after 24 h of pretreatment with 5 mol/L NaOH solution for 14 days.
出处
《骨科》
CAS
2010年第2期69-73,共5页
ORTHOPAEDICS
基金
国家高技术研究发展计划(863计划)(课题编号:2006AA02A136)
关键词
连通多孔钛
类骨磷灰石
模拟体液
生物矿化
Interconnected porous titanium
Bonelike apatite
Simulated body fluid
Biomineralization
