摘要
目的观察维生素Ｅ对肿瘤坏死因子（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）损伤内皮细胞的保护作用，探讨维生素Ｅ抗动脉粥样硬化形成的可能机理。方法以体外培养的小牛主动脉内皮细胞为模型，在培养的内皮细胞中加入不同剂量的维生素Ｅ和浓度为４００Ｕ／ｍｌ的ＴＮＦα共同培养７２ｈ，收集培养的内皮细胞，通过噻唑蓝比色分析法、流式细胞仪分析法和单核细胞（ＨＬ６０）粘附计数法对其进行检测。结果加维生素Ｅ组内皮细胞形态损伤程度明显轻于ＴＮＦ组；其内皮细胞粘附的单核细胞数亦显著少于ＴＮＦ组；流式细胞仪分析法及噻唑蓝比色分析法检测结果表明维生素Ｅ可减轻ＴＮＦ对细胞生长增殖的抑制作用、促进细胞增殖即促进内皮细胞由Ｇ１期进入ＤＮＡ合成的Ｓ期、抑制内皮细胞凋亡。结论维生素Ｅ可明显减轻ＴＮＦ对内皮细胞的损伤作用，并可能与ＶＥ促进内皮细胞增殖、抑制凋亡及阻止单核细胞粘附有关。维生素阻抑ＴＮＦ对内皮细胞损伤作用可能是维生素Ｅ抗动脉粥样硬化形成的重要机制之一。
Objective To explore the possible mechanism of vitamin E against atherosclerosis through its preventive effect on tumor necrosis factor (TNF) inducing endothelial cell injury.Methods Bovine aortic endothelial cells were incubated in culture media with TNF alpha (400 U/ml) and vitamin E (12.5 microM, 25.0 microM and 50.0 microM respectively) for 72 h.The morphology, proliferation, apoptosis and adherence of endothelial cells were investigated with phase contrast microscope, MTT method, flow cytometry analysis and cell count respectively.Results Compared with TNF group, the phenomena of endothelial cell deformity and shedding were slighter, the number of adhered HL60 monocyte cells and G 1 phase cells were less, and the inhibitory rate and the apoptotic peaks were significantly lower in vitamin E groups.Conclusion The results suggested that vitamin E has definite protective effects on injury induced by TNF as shown in morphology, proliferation and apoptosis of endothelial cells in vitro .
基金
国家自然科学基金
关键词
动脉粥样硬化
维生素E
内皮细胞
肿瘤坏死因子
vitamin E
endothelial cell
tumor necrosis factor
proliferation
apoptosis
