摘要
以人工重组ＴＳＨ受体表达的中国仓鼠卵细胞（ＣＨＯ－ＴＳＨＲ）为靶细胞，用流式细胞（ＦＡＣＳ）测定技术，对４５例自身免疫性甲状腺病（ＡＩＴＤ）患者血清中ＳＨ受体抗体（ＴＲＡｂ）进行测定。结果显示：３６例ＧＤ患者阳性细胞结合的百分数为１．９％～７７．４％。阳性率为９１．６６％。其均值２６．１３９％与正常人均值６．１６３％相比有显著性差异（Ｐ＝０．０００１）。９例桥本（ＨＴ）甲减患者５例阳性，阳性率为５５．５６％。证实以ＣＨＯ－ＴＳＨＲ细胞为靶细胞用ＦＡＣＳ进行ＴＲＡｂ汕测定不失为一种敏感简便的新方法。
Using Chinese hamster ovary(CHO) cells that express high numbers of TSH receptor (TSHR) on the surface, we studied bindingactivity of antibodies in patients' sera to the TSHRby flow cytometry. It showed thatthe binding activity of 36 sera from active Graves' disease patientswas. 1.9%~77. 4%, while 30 sera from normalsubjects was 0. 1% ~15. 1 %. The positive rate was91. 66%. The median binding activity in activeGraves' disease patients (26. 139% ) was significantly higher than that of normal subjects (6. 163%;P=0. 0001). We also studied 9 sera from Hoshimototyroiditis (HT). The positive rate was 55. 56%. Inconclusion, this method can be used to detect binding of antibodies to TSHR.
出处
《西安医科大学学报》
CSCD
1999年第1期115-117,共3页
Journal of Xi'an Medical University(Chinese)
关键词
甲状腺疾病
AITD
流式细胞仪
TSH受体抗体
TSH receptor antibodies (TRAb)
flow cytometric analyses (FACS)
Chinese hamster ovarycells-TSH receptor (CHO-TSHR)
Gravea' disease (GD)
