摘要
目的研究鼻咽癌细胞系ＳＵＮＥ中ＥＢＶ┐ＬＭＰ１基因对上皮细胞增殖的影响，探索ＬＭＰ１在鼻咽癌发生中所起的作用。方法用ＬＭＰ１基因真核表达质粒转染人胚肾上皮细胞，检测ＬＭＰ１的表达，观察细胞在软琼脂中的集落形成能力，ＭＴＴ吸收能力以及ＰＣＮＡ的表达情况。结果被ＬＭＰ１基因转染的细胞生长旺盛，能在软琼脂中形成多个集落，ＭＴＴ吸收能力增强，ＰＣＮＡ的表达水平增高。结论ＬＭＰ１基因能明显改变上皮细胞的生物学行为，促进细胞的生长、增殖和转化，使转染的上皮细胞获得肿瘤细胞的生长特征。
Objective:To study the influence of EBV latent membrane protein1 (LMP1) gene isolated from nasopharyngeal carcinoma cell line SUNE on the proliferation of epithelial cells and the role of LMP1 in tumorigenesis of nasopharyngeal carcinoma.Methods:EBV LMP1 gene eukaryotic expression plasmid was transfected into human embryo kidney epithelial cell.The expression of LMP1 protein in transfected cells was detected.The formation of colonies in soft agar,the value of MTT absorbed and the expression of PCNA were observed.Results:The transfected cells grew vigorously and formed more colonies in soft agar,the MTT uptake and PCNA expression were apparently increased.Conclusion:LMP1 gene could alter the biological behavior of epithelial cells,promote cell growth,proliferation and transformation,and endow the transfected cells with growth characteristics like tumor cells.
出处
《肿瘤》
CAS
CSCD
北大核心
1999年第1期15-18,共4页
Tumor
基金
美国中华医学基金
关键词
EB病毒
潜伏膜蛋白1
上皮细胞增殖
鼻咽癌
Epstien Barr virus Latent membrane protein 1 Epithelial cell,proliferation
