摘要
目的建立测定人血浆中兰索拉唑(抗胃及十二指肠溃疡药)含量的超快速液相色谱-质谱/质谱联用法(UFLC-MS/MS)。方法血浆样品经甲醇沉淀蛋白后,以乙腈-0.1%甲酸(27∶73)洗脱,用UltimateXB-CN(5μm,2.1mm×150mm)色谱柱,通过电喷雾离子化三重四级杆串联质谱,经多反应监测模式检测,m/z370.4→m/z252.2(兰索拉唑),m/z237.2→m/z194.3(卡马西平,内标)。结果兰索拉唑在5.01～5007.90μg.L-1内线性关系良好(γ=0.9985),最低定量浓度为5.01μg.L-1。方法回收率(n=5)分别为90.21%,89.71%,92.27%,日内和日间精密度均<9%。结论该法操作简单,灵敏,准确,重现性好,适用于该药的临床药代动力学研究。
Objective To establish an UFLC - MS/MS method for the determination of lansoprazole concentration in human plasma. Methods The plasma was deproteinated by methanol. The separation was performed on an Ultimate XB - CN （5 μm ,2. 1 mm × 150 mm） columm. The mobile phase consisted of acetonitrile - water （27：73 ） containing 0. 1% formic acid was used with the gradient elution. Detection was performed on a tandem mass spectrometry with electrospray ionization sourse in positive ion mode. Multiple reaction monitoring（MRM） mode with the transition of m/z 370. 4-→m/z 252.2 （ lansoprazole ） and m/z 237.2→m/z 194, 3 （carbamazepine） was used to quantify lansoprazole and internal standard respeetively. Results The linear range was 5. 01 - 5007.90 μg · L^-1 ,and the limit of quantitation was 5.01 μg · L^-1. The method recoveries were 90.21% ,89.71% ,92.27% respectively and the RSD of inter - day and intra - day was less than 9%. Conclusion The method is simple, sensitive, aceurate and reproducible. It is applicable for pharmacokinetic study of lansoprazole.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2010年第7期525-528,共4页
The Chinese Journal of Clinical Pharmacology
