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实时荧光RT-PCR检测儿童流感病毒

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摘要 目的对湛江地区的流感病毒感染情况进行调查,采用实时荧光RT-PCR技术快速检测流感病毒,用于流感的诊断。方法收集湛江地区2009年具有流感样症状患者的鼻咽拭子等标本351份,采用实时荧光RT-PCR技术检测标本中的流感病毒,与酶免疫分析技术(EIA)方法进行对照。结果 351例标本中,A、B型流感病毒阳性检出率分别为8.3%(29/351)和3.1%(11/351),本方法与EIA法具有较高的相关性和符合率,均可用于流感病毒的早期诊断。结论实时荧光RT-PCR技术均可用于儿童流感病毒的临床诊断。
出处 《齐齐哈尔医学院学报》 2010年第11期1772-1773,共2页 Journal of Qiqihar Medical University
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