摘要
利用树脂纯化与结晶法技术相结合,从大豆糖蜜中分离制备高纯度大豆皂苷。研究了固形物质量分数、温度、离心转速、离心时间对大豆皂苷含量和收率的影响,通过正交实验对工艺进行优化,确定最佳工艺条件为:大豆糖蜜经H103树脂纯化,解吸液回收乙醇并浓缩至固形物质量分数为3%,在95℃、3 500 r/min下离心7.5 min,沉淀经干燥制得的产品中大豆皂苷含量达94.25%,收率为51.26%。
High -purity soybean saponins was produced from soybean molasses by resin purification and crystallization method. The effects of various influence factors on the purity and yield of soybean saponins were discussed. The optimum conditions determined by orthogonal experiment were that soybean molasses was purified by H103 resin and the desorption solution was heated to remove the eluent of alcohol and concentrated to solid mass fraction 3 %, then the concentrate was centrifuged for 7.5 min at 95℃ and 3 500 r/min, and the sediment was dried to obtain the product of soybean saponins. The purity of soybean saponins was 94.25% and the yield was 51.26%.
出处
《中国油脂》
CAS
CSCD
北大核心
2010年第7期58-60,共3页
China Oils and Fats
关键词
大豆皂苷
H103树脂
结晶法
离心
高纯度
soybean saponin
H103 resin
crystallization method
centrifugation
high - purity
