摘要
目的：采用反相高效液相色谱法测定４种人工冬虫夏草中腺甘的含量。方法：采用ＧｉｌｓｏｎＨＰＬＣ系统，ＤｕｐｏｎｔＣ１８色谱柱，甲醇０．０６ｍｏｌ／Ｌ磷酸二氢钾四氢呋喃（１０∶１５０∶１．５）为流动相，检测波长为１６０ｎｍ，流速１ｍｌ／ｍｉｎ。结果：该法平均回收率９８．７％，相对标准偏差ＲＳＤ为１．１０％（ｎ＝５）。结论：该法快速简便、灵敏度高。
Objective:A method for determination of adenosine in four fermented Cordyceps products by RP HPLC is described.Method:C 18 column was used with methanol 0.06mol/L potassium phosphate monobasic tetrahydrofuran(10∶150∶1.5)as mobile phase.The flow rate was 1ml/min and detected at 260nm.Results: The average recovery was 98.7% and relative standard deviation was 1.10%(n=5).Conclusion:This method is simple,rapid and sensitive.The paper introduces the feasibility of supersonic extraction method taking the place of reflux method.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1999年第1期12-14,共3页
China Journal of Chinese Materia Medica
