摘要
本研究旨在筛选牛体外受精胚胎早期发育差异表达基因,并进行差异表达分析。选取牛卵母细胞、体外受精8细胞期和囊胚期胚胎为试验材料,进行mRNA差异表达研究。初步克隆出4个牛卵母细胞和早期胚胎发育不同阶段差异表达的基因,同源性分析显示,它们分别与高移动样蛋白盒结构域4基因(HMGXB4)、热休克蛋白40亚家族C成员8基因(Dnajc8)、突触膜胞吐调节2基因(RI MS2)和核糖体蛋白L31基因(RPL31)高度同源。RT-PCR检测验证,HMGXB4、Dnajc8、RI MS2和RPL31在牛卵母细胞和早期胚胎发育过程中mRNA表达量存在时间性差异,可能与其参与不同的生理活动有关。
This experiment was conducted to screen differential expressed genes of bovine preimplantation embryos derived from in vitro fertilization and make differential expression analysis.Bovine oocytes,8 cell stage embryos and blastocysts derived from in vitro fertilization were selected for mRNA differential display in this study.Four differential expression genes in bovine oocytes and preimplantation embryos in total were screened.The sequencing and alignment results indicated that 4 differential expression genes were highly homologous with HMGXB4,Dnajc8,RIMS2 and RPL31.The results of RT-PCR detection showed that genes expression of HMGXB4,Dnajc8,RIMS2 and RPL31 in bovine oocytes and preimplantation embryos were temporal-differential,and the reasons might be related with participating in different physiological activities.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第7期816-822,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家基础科学人才培养基金(J0730648)
国家自然科学基金(30760168)
内蒙古自然科学基金重大项目(200711020401)资助
关键词
牛
早期胚胎
MRNA差异显示
基因表达
bovine
preimplantation embryos
mRNA differential display
gene expression
