摘要
为了解柳州和南宁两市HIV-1亚型分布和耐药情况,在柳州和南宁招募HIV感染者和AIDS患者共304名,采集外周静脉血,从血浆中提取HIVRNA,扩增HIVpol基因并测序。将获得的序列进行系统进化树分析,结果表明柳州的HIV-1毒株中存在CRF01_AE和CRF07_BC两种亚型,其中CRF01_AE毒株占75.2%,CRF07_BC毒株占24.8%;南宁的HIV-1毒株中存在CRF01_AE、CRF08_BC、B亚型和C亚型共4种亚型,其中CRF01_AE和CRF08_BC仍是南宁最主要的亚型,CRF01_AE占85.8%,CRF08_BC占11.5%。根据所得的序列资料进行HIV-1耐药性分析,计算耐药率。计算结果表明,柳州未治疗和治疗研究对象的耐药率分别为3.3%和8.7%,南宁未治疗和治疗研究对象的耐药率分别为1.4%和27.5%。
To investigate HIV-1 subtype distribution and prevalence of HIV-1 drug resistance in Liuzhou and Nanning,a total of 304 HIV-infected subjects or AIDS patients from Liuzhou and Nanning were recruited.Whole blood was withdrawn from a peripheral vein of each subject.HIV RNA were extracted from plasma,and subjected to PCR amplification targeting HIV pol gene fragment and DNA sequencing.Sequences obtained were subtyped by phylogenetic analysis.Two subtypes,CRF01_AE and CRF07_BC,were found in subjects from Liuzhou,accounting for 75.2% and 24.8%,respectively.Subtype CRF01_AE,CRF08_BC,B,and C were found in subjects from Nanning.CRF01_AE and CRF08_BC were still the dominant strains in Nanning,accounting for 85.8% and 11.5%,respectively.Sequences were also analyzed for drug resistance mutations,and rates of drug resistance were calculated.The rate of drug resistance was 3.3% in ART-naive subjects from Liuzhou,and 8.7% in the ART-experienced.For patients from Nanning,the rate was 1.4% in ART-naive subjects,and 27.5% in ART-experienced subjects.
出处
《病毒学报》
CAS
CSCD
北大核心
2010年第4期290-294,共5页
Chinese Journal of Virology
基金
国家自然科学基金项目(30760218)
广西卫生厅重点科研课题(200628)
关键词
人类免疫缺陷病毒1型
亚型
耐药性
human immunodeficiency virus type 1
subtype
drug resistance
