摘要
目的探讨中药循经敷贴治疗哮喘的作用机制。方法采用Hutson法豚鼠模型,治疗组隔日诱喘后用中药敷贴30min;对照组隔日诱喘后采用生理盐水浸润纱布敷贴,时间同治疗组。记录各组诱喘潜伏期时间。2周后处死豚鼠,取肺组织,用半定量RT-PCR法比较Eotaxin mRNA、IL-5 mRNA的表达。结果中药循经敷贴能显著延长豚鼠诱喘潜伏期,下调哮喘豚鼠肺组织Eotaxin mRNA、IL-5 mRNA的表达。结论抑制变异性炎症可能是中药循经敷贴治疗哮喘的重要机制之一。
AIM To investigate the mechanism of external application of Chinese herbs on allergic asthma. METHODS The asthma guinea pig models induced by Huston＇ s methods were externally applied with Chinese herb for 30 min, qod. A control group of guinea pigs were applied with wet gauzes in 9 g· L^- 1 normal saline in same times . The arousal time was compared and signed. The lung tissues were got after they were killed 2 weeks later. The method of RT- PCR was used to compare the expression of Eotaxin mRNA and IL-5 mRNA. RESULTS The arousal time could be prolonged significantly and the expression of Eotaxin mRNA and IL-5 mRNA could be fallen through the external application of Chinese herbs. CONCLUSION The anti-inflammation factors may be the one of the important mechanism of herbal penetration therapy on allergic asthma.
出处
《中国临床药学杂志》
CAS
2010年第4期222-225,共4页
Chinese Journal of Clinical Pharmacy
基金
上海市科学技术发展基金资助(编号024119084)
