摘要
背景:司坦唑醇能提高骨质疏松患者骨密度,具有促进骨形成和抑制骨吸收作用,但目前还没有报道显示其对老年性骨质疏松的效果如何,对不同部位骨骼的影响也未见报道。目的:通过骨形态计量学观察复方司坦唑醇对D-半乳糖大鼠骨质疏松模型不同部位骨骼的影响。方法:将SD大鼠以数字表法随机分为正常对照组、D-半乳糖模型组、复方司坦唑醇组。除正常对照组颈背部皮下注射生理盐水外,其余两组颈背部皮下注射D-半乳糖制备骨质疏松模型。正常对照组和D-半乳糖模型组灌胃给予溶剂对照,复方司坦唑醇组灌胃给予司坦唑醇0.54mg/(kg·d)+吡拉西坦432mg/(kg·d),连续14周。测量胫骨上段松质骨和胫骨中段皮质骨骨组织形态计量学参数。结果与结论:骨组织形态计量学参数显示,复方司坦唑醇可有效预防D-半乳糖对大鼠胫骨上段松质骨显微结构的破坏,抑制骨吸收,促进骨形成。复方司坦唑醇对D-半乳糖大鼠胫骨中段的皮质骨骨量丢失的作用不大,可抑制D-半乳糖大鼠皮质骨骨外膜的骨形成。
BACKGROUND： Stanozolol can increase bone mineral density in osteoporosis patients, which promotes bone formation and inhibiting bone resorption, however, its effectiveness in senile osteoporosis, as well as its effects on the different parts of the skeleton, remains unclearly. OBJECTIVE： To study the effect of compound stanozolol on different skeletal sites of rat D-galactose-induced osteoporosis model through bone histomorphometry observation. METHODS： Sprague Dawley rats were divided into the normal control, D-galactose and compound stanozolol groups according to random number table. All rats were prepared osteoporosis models by subcutaneous injecting D-galactose exception of the normal control group. In the compound stanozolol group, rats were gastric irrigated with stanozolol 0.54 mg/（kg·d）＋Piracetam 432 mg/（kg·d） for 14 successive weeks. Solvent control was performed between normal control and D-galactose group. Bone histomorphometric parameter of the proximal tibial metaphysis （PTM）, and tibial shaft was calculated. The biomechanic properties of right femur were analyzed by three-point bending test. RESULTS AND CONCLUSION： Bone histomorphometric analysis showed that compound stanozolol could prevent micro-structural damage of PTM caused by D-galactose, inhibit bone resorption and facilitate proximal tibial bone formation. However, compound stanozolol had no obviously effect on cortical bone mass loss of tibial shaft by D-galactose.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第28期5141-5145,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
湛江市科技计划项目(2008C04016)~~
