摘要
目的:利用离子导入仪将钙离子导入至离体乳牙中,运用电子探针检测离子导入前后乳牙釉质中钙元素含量变化,为我国儿童乳牙龋病防治提供参考。方法:选取5～7岁健康儿童因滞留拔除的下颌无龋乳中切牙20个,沿牙体长轴唇舌向正中对半切开,分别作为实验组与对照组。实验组用离子导入仪,以150g/L氯化钙液为导入液进行钙离子导入处理,每次20min,每日1次,连续处理10d。对照组不做任何处理。用电子探针微量分析法测量两组釉质表层下不同深度处钙元素含量,并作统计分析。结果:釉质表层下10、20、30、50、100μm各层钙元素含量,实验组均明显高于对照组(P<0.05);而在150、200、250μm各层的钙元素含量与对照组相近,差异无统计学意义(P>0.05)。釉质表层下10～100μm6个不同深度之间钙元素增值比较,釉质表层下50μm内的每10μm深度各层钙元素增加值相近(P>0.05),100μm处明显降低,与以上各层相比均有显著性差异(P<0.05)。结论:钙离子导入可明显增强乳牙牙釉质表层钙元素含量,提示离子导入法在儿童龋病防治方面具有潜在的应用价值。
AIM ： To study the alterations of calcium in enamel of deciduous teeth after sodium calcium ion- tophoresis. METHODS： A total of 20 lower deciduous central incisors were selected from 5-7 years old caries-free children. All teeth were sectioned into halves, one half as test group and the other as the control group. The test group was treated with CaC12 solution （15% Ca2~ ） with iontophoresis for 20 min, once daily for 10 consecutive days. The control group received no treatment. Calcium concentrations in enamel were measured by electron probe microanalysis （EPMA） point analysis. RESULTS： Calcium concentrations in the test group at 10 ~m, 20 p^m, 30 p,m, 50 p.m and 100 p,m from enamel surface were significantly higher those in the control group （P 〈0.05 ）. However, no significant differences were observed between the test and control group at 150 ~Lm, 200 p^m and 250 p^m from enamel surface （P 〉0.05）. CONCLUSION： Calcium iontophoresis method can increase the calcium concentration of the enamel surface in deciduous teeth. It implies that calcium iontophoresis might have potential value in caries prevention.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2010年第7期390-392,399,共4页
Chinese Journal of Conservative Dentistry
关键词
钙元素
釉质
乳牙
离子导入
电子探针
calcium
enamel
deciduous teeth
iontophoresis
electron probe
