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黄瓜营养体苦味基因Bi的定位 被引量:14

Fine Mapping of the Foliage Bitterness Gene(Bi)in Cucumis sativus
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摘要 以黄瓜(Cucumis sativusL.)营养体无苦味的材料9110Gt(bibi)与有苦味的材料9930(BiBi)为亲本,对以两亲本构建的148个株系的RILs群体进行SSR标记的遗传连锁分析,对Bi基因进行初步定位,两侧翼标记SSR19672和SSR00004与Bi基因的连锁距离分别为4.4和3.4cM。利用两侧翼标记筛选以相同亲本构建的1815株的F2群体中的重组单株,结合黄瓜基因组测序的结果及生物信息学的知识,在标记区域内开发了31对SSR标记,最终将Bi基因定位在黄瓜的第6染色体上,最近的两侧翼标记SSR02309和SSR00004与苦味基因分别相距1.7和2.2cM。 A RILs population with 148 individuals derived from a cucumber line 9110 Gt(bibi)and 9930(BiBi)was used to analyze the location of Bi gene.Bi gene was mapped on the linkage group 6,corresponding to chromosome 6 of cucumber.The flanking markers SSR19672 and SSR00004 were linked to the Bi gene with distances of 4.4 and 3.4 cM.An F2 population with 1 815 individuals derived from the same parents was used for screening recombinants for fine mapping of the gene.Recombinant plants were identified by the flanking markers SSR19672 and SSR00004.Based on the whole genome sequence of cucumber,thirty-one SSRs within the region between SSR19672 and SSR00004 were designed and screened for polymorphism between the two parents,which revealed that one of them(SSR02309)was 1.7 cM to Bi gene.The distance between previously identified flanking marker SSR00004 and Bi were 2.2 cM.
出处 《园艺学报》 CAS CSCD 北大核心 2010年第7期1073-1078,共6页 Acta Horticulturae Sinica
基金 农业部园艺作物遗传改良重点开放实验室项目
关键词 黄瓜 营养体 苦味 Bi基因 基因定位 SSR cucumber foliage bitterness Bi gene gene mapping SSR
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参考文献13

  • 1Andeweg J M, De Bruyn J W. 1959. Breeding of non-bitter cucumbers. Euphytica, 8.. 13 - 20.
  • 2Barham W S. 1953. The inheritance of a bitter principle in cucumbers. Proc Amer Soc Hort Sci, 62:441 - 442.
  • 3池秀蓉,顾兴芳,张圣平,王晓武,王烨.黄瓜无苦味基因bi的分子标记研究[J].园艺学报,2007,34(5):1177-1182. 被引量:13
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二级参考文献17

  • 1顾兴芳,方秀娟,张孟玉,张天明.黄瓜苦味研究概况[J].园艺学报,2000,27(z1):504-508. 被引量:10
  • 2顾兴芳,张圣平,国艳梅,徐彩清.黄瓜苦味遗传分析[J].园艺学报,2004,31(5):613-616. 被引量:15
  • 3Andeweg J M, DeBruyn J W.Breeding of non-bitter cucumbers.Euphytica, 1959, 8:13~20
  • 4Lawrence K P, Wehner T C. Review of genes and linkage groups in cucumber. Hortscience, 1990, 25(6) :605~615
  • 5Barham W S. The inheritance of a bitter principle in cucumbers.Proc. Amer. Soc. Hort. Sci., 1953, 62 : 441~442
  • 6Wehner T C, Liu J S. Two-gene interaction and linkage for bitterfree foliage in cucumber.J. Amer. Soc. Hort. Sci., 1998, 123(3):401~403
  • 7Walters S A, Shetty N V, Wehner T C. Segregation and linkage of several genes in cucumber. J.Amer.Soc.Hort.Sci., 2001, 126(4): 442~450
  • 8Walters S A, Wehner T C. Independence of the mj nematode resistance gene from 17 gene loci in cucumber.Hortscience, 1998, 33(6): 1050~1052
  • 9Andeweg J M,DeBruyn J W.1959.Breeding of non-bitter cucumbers.Euphytica,8:13-20.
  • 10Barham W S.1953.The inheritance of a bitter principle in cucumbers.Proc.Amer.Soc.Hort.Sci.,62:441-442.

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