摘要
应用大孔吸附树脂对杜仲叶超临界法提取液中的黄酮类物质进行富集和纯化,得到树脂富集杜仲叶黄酮的最优工艺条件。对4种大孔吸附树脂NKA-2、X-5、D101、AB-8的吸附和解吸能力进行比较的结果表明:AB-8树脂的吸附率和解吸率都最高,最佳吸附洗脱工艺为上样液黄酮质量浓度193.92mg/mL、pH2、吸附流速2.6mL/min、洗脱流速1.6mL/min、解吸剂80%乙醇用量30mL。所得洗脱液中黄酮质量分数从纯化前的10.2%可增加到纯化后的42.6%以上。
In this study,the leaves of Eucommia ulmoides Oliv.were extracted with absolute ethanol to obtain flavonoid compounds.The crude extract was purified using 4 macroporous adsorption resins,and AB-8 was found to be the best choice due to both the highest adsorption and desorption capacity.The optimum conditions for adorption/desorption of the crude extract of the leaves of Eucommia ulmoides Oliv.were as follows： sample concentration 193.92 mg/mL at pH 2,sample flow rate 2.6 mL/min,desorption flow rate 1.6 mL/min and amount of 80% ethanol required for desorption 30 mL.After the optimized purification,the concentration of flavonoids was improved from 10.2% to 42.6%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第14期67-70,共4页
Food Science
基金
江西省自然科学基金项目(2008GQN0042)
关键词
杜仲叶黄酮
富集纯化
大孔吸附树脂
Eucommia ulmoides leaf flavonoids
enrichment and purification
macroporous resin
