摘要
为从大豆异黄酮降解液中分离纯化雌马酚,以雌马酚的含量为指标,确定聚酰胺树脂为吸附剂纯化雌马酚。通过单因素试验和正交试验,确定最佳吸附条件为粗提液中雌马酚质量浓度2.40μg/mL、pH5、吸附时间6h,得雌马酚吸附率45.36%,最佳解吸条件为乙醇溶液体积分数80%、乙醇溶液用量45mL、乙醇溶液流速0.5mL/min,得解吸率60%。
MRS medium fortified with soybean isoflavones was fermented by Lactobacillus delbrueckii subsp.Bulgaricus at 37 ℃.As a result,soybean isoflavones were degraded,producing equol.Polyamide resin was used for purifying the crude equol extract from the harvested fermentation broth after 48 h fermentation.The optimal adsorption/desorption conditions were determined by single factor and orthogonal array design methods to be： sample concentration 2.40 μg/mL at pH 5,adsorption duration 6 h and amount of 80% ethanol used for equol desorption at a flow rate of 0.5 mL/min 45 mL.Under such conditions,the adsorption ratio of equol was 45.36%,and the desorption raio 60.00%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第14期128-131,共4页
Food Science
关键词
雌马酚
聚酰胺
纯化
equol
polyamide
purification
