摘要
目的使用随机扩增多态DNA标记建立标准化的布氏田鼠封闭群遗传质量控制分子标记库。方法使用高盐沉淀法从鼠尾中提取布氏田鼠基因组DNA。采用40条PRAD引物对布氏田鼠封闭群进行PCR扩增,琼脂糖电泳分离条带,参考标准分子量标记计算条带大小,并使用多态位点数、单态位点数以及多态位点比率评价种群的遗传多样性。结果筛选出8个能获得清晰稳定扩增条带的RAPD标记。这8个RAPD标记检测到的多态位点数存在明显差异。8个引物得到的遗传多态位点的数据之和能揭示种群的遗传结构。结论本实验建立了检测布氏田鼠封闭群遗传结构的RAPD标记。
Objective To establish the he genetic structure for cultivation population of Brandt＇s vole （Lasiopodomys brandtii） by RAPD （randomly amplified polymorphic DNA） assay. Method Genomic DNA was extracted from tail tip following high-concentration-salt precipitation methods. 40 RAPD primers were PCR amplified to analyze the DNA structure of Brandt＇s vole population. RAPD markers were separated by agarose gel electrophoresis. Alleles were determined according to a size marker. Monomorphic site,polymorphic site and the proportion of polymorphic loci,were used to describe the genetic variation of Brandt＇s vole population detected by RAPD. Results The rates of variation of RAPD loci by 8 primers used in this study are different. The combination of the PCR bandings by eight RAPD primers can provide sufficient statistical power to assess the genetic variations in Brandt＇s vole population. Conclusion We have successfully established genetic methods for analyzing genetic structure of closed colony of Brandt＇s vole.
出处
《中国比较医学杂志》
CAS
2010年第7期49-52,共4页
Chinese Journal of Comparative Medicine
基金
高等学校博士学科点专项科研基金(20070023119)
中央级公益性科研院所基本科研业务费专项基金(DWS200702)
国家科技支撑计划课题(2009BAI83B01)子课题资助
