摘要
以强德勒红心柚(Citrus grandis Osbeckcv. Chandler)种子萌发的无菌苗为材料,选取子叶、子叶节段、上胚轴、带芽的茎段进行离体培养研究。结果表明:子叶节段是诱导丛生芽的最佳外植体,诱导率100%;诱导丛生芽的最佳培养基为MS+6-BA2.0mg/L+NAA0.05mg/L+蔗糖30g/L+活性炭0.4g/L,丛生芽增殖可达11.2倍;最适生根培养基为1/2MS+NAA0.5mg/L,生根率达100%,移栽15d后成活率100%。
The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction（induction rate 100%）.The best medium for inducing bush-bud was MS＋6-BA 2.0 mg/L＋NAA 0.05 mg/L＋sucrose 30 g/L＋activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS＋NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.
出处
《武汉植物学研究》
CAS
CSCD
北大核心
2010年第2期218-223,共6页
Journal of Wuhan Botanical Research
基金
四川省教育厅项目(2003A175)
四川省绵阳市科技攻关项目(2005BN001-1)
关键词
强德勒红心柚
离体培养
丛生芽增殖
Citrus grandis Osbeck cv.Chandler
Culture in vitro
Bush-bud regeneration
