摘要
[目的]探讨水杨酸盐分光光度法测定空气中氨的方法。[方法]用装有酸性溶液的大型气泡吸收管采集空气样品,以水杨酸盐为显色剂,采用722N型分光光度计,于波长690 nm处测定光密度比色定量。[结果]该方法的检出限为0.02μg/mL,日间精密度和日内精密度的相对标准偏差为0.5%-2.1%,样品加标回收率在96.0%-103.0%之间。标准参考物质的测定值在标准值范围内,测定结果与国标法（纳氏试剂分光光度法）比较,差异无统计学意义（P〉0.05）。[结论]空气中氨的水杨酸盐分光光度法操作简单,干扰少,具有良好的选择性和重现性,适合在基层推广应用。
[Objective] To develop an approach to determining ammonia in air by salicylate spectrophotometry.[Methods] To collect air samples by large air-bubble absorption tube containing acidic solution;apply salicylate as chromogenic reagent and use 722N spectrophotometer in 690 nm wave-length to measure absorbency;determine the quantities of ammonia in air samples by colorimetric analysis.[Results] The detection limit of this approach was 0.02 μg/mL;the relative standard deviations between inter-day precision and intra-day precision ranged between 0.5% to 2.1%;the spike recovery rates of samples ranged from 96.0% to 103.0%.The determined results of standard samples were within standard range.There were no significant differences between the results determined by salicylate spectrophotometry and GB method（Nessler＇s reagent method）（P〉 0.05）.[Conclusion] The approach to determining ammonia in air by salicylate spectrophotometry is easily operated and less interfered.Therefore,it can be easily selected and replicated at the grassroots level.
出处
《环境与职业医学》
CAS
北大核心
2010年第7期429-431,共3页
Journal of Environmental and Occupational Medicine
