摘要
采用实验生态学方法研究了不同盐度波动幅度对凡纳滨对虾(Litopenaeus vannamei)稚虾蜕皮、耗氧率、排氨率和排尿率的影响。实验以D_1蜕皮期的对虾为实验材料,虾的初始湿体重为(2.485±0.303)g,实验设盐度29为对照组(用C表示),4个不同的盐度波动幅度(分别为2、4、6、8)为处理组,分别用S2、S4、S6和S8表示,每个处理设10个重复。实验分别于降低盐度和恢复盐度至29的不同时间点采样,测定对虾的耗氧率、氨氮和尿素的排泄率,实验持续7 d。主要实验结果如下:(1)降低盐度后,S4组、S6组及S8组对虾的蜕皮整体提前1 d,且S6组对虾的蜕皮较为集中;(2)降低盐度后,所有处理组对虾耗氧率均呈现先下降后随着蜕皮到来逐渐升高的趋势,且降盐后S4组和S6组对虾耗氧率的下降速度最快(6 h),在升盐过程中,仅对照组在6 h和S4组在6 h和12 h处对虾的耗氧率与升盐前相比差异达到显著水平(P<0.05);(3)对虾的排氨率呈现出盐度波动后下降的趋势(S8组除外),并随蜕皮期的到来升高(S4组除外),在整个实验过程中S4组、S6组对虾的排氨率较低,S8组对虾的排氨率较高;(4)对照组和S8组对虾的排尿率同样表现出与蜕皮高峰相吻合的关系,而S2组、S4组及S6组对虾的排尿率在降盐24 h后趋于稳定。
This experiment was conducted to investigate the effects of salinity fluctuation on the molt, oxygen consumption rate, ammonia-N excretion rate and urea-N excretion rate of juvenile Litopenaeus vannarnei with an initial wet body weight of （2. 485±0. 303） g. The salinity of the control treatment （C） was 29 throughout the experiment, while treatment S2, S4, S6 and S8 groups were subjected to different salinity fluctuation and the range was 2, 4, 6 and 8, respectively. Each treatment group had 10 replicates. The salinity was declined at Stage Dlof the shrimp, then samples were taken at different times. The salinity was increased to 29 at 97th hour, and re-sampled. The entire experiment lasted 7 days. The test shrimps in treatment S4, S6 and S8 groups molted one day ahead of schedule after salinity decrease, and more concentrated molt occurred in S6 treatment. The oxygen consumption rate of the test shrimps showed a decrease trend first, and increased gradually with the arrival of the molt stage in treatment C, S4, S6 and S8 groups. The oxygen consumption rate of treatments C and S4 were significantly lower than those at 96^th hour after the salinity increase. Ammonia excretion rate of the test shrimps also presented a decrease trend after the salinity fluctuation （except treatment S8）. The urea-N excretion rates of the test shrimps in treatment S2, S4 and S6 groups were relatively stable after 24 hours of salinity decrease. And the peaks of the oxygen consumption rate, ammonia excretion rate and urea-N excretion rate in treatment C and S8 groups occurred and coincided with the molt peak during the experiment.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第7期47-52,共6页
Periodical of Ocean University of China
基金
国家科技支撑计划项目(2006BAD09A07)
山东省优秀中青年科学家奖励基金项目(2006BS07002)
关键词
凡纳滨对虾
盐度突变
蜕皮
耗氧率
排氨率
排尿率
Litopenaeus vannamei
salinity
molt
oxygen consumption rate
ammonia-N excretion rate
urea-N excretion rate
