摘要
目的探讨宫颈癌的发生发展与人乳头状瘤病毒（ＨＰＶ）和人巨细胞病毒（ＨＣＭＶ）协同感染的关系。方法应用我室建立的ＨＰＶ１６永生化的人宫颈上皮细胞系，转染ＨＣＭＶ即早基因（ＩＥ）。结果ＨＣＭＶ整合在细胞染色体中，协同ＨＰＶ１６促进宫颈上皮细胞的恶性转化，在裸鼠中形成肿瘤。结论ＨＣＭＶＩＥ基因可协同ＨＰＶ１６恶性转化宫颈上皮细胞，并对其协同机制进行了细胞和分子生物学的探讨。
Objective The carcinogenesis of the human cervical carcinoma is known closely associated with human papillomavirus (HPV). The purpose of this article is to identify whether another sexually transmitted factor, human cytomegalovirus (HCMV) plays as a co factor role in the carcinogenesis. Methods Human cervical epithelial cells immortalized by HPV16 were transfected with IE gene of HCMV. The condition and efficiency of IE gene were examined. Results It was showed that the cervical epithelial cells could be completely transformed into malignant by HPV 16 and IE gene of HCMV and tumor was formed by injecting the transformed cells in nude mice. Conclusion The co operation effect of HPV and HCMV auailed the oncogenesis of human cervical carcinoma and moreover, the cellular and molecular biological mechanism was discussed.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1999年第1期24-27,共4页
Chinese Journal of Pathology
关键词
子宫颈肿瘤
HPV
HCMV
癌变
上皮细胞
Cervix neoplasms Papillomavirus, human Cytomegalovirus infection Tumor cells, Cultured
