摘要
目的：探讨肝细胞癌患者血液甲胎蛋白（ＡＦＰ）ｍＲＮＡ检测的临床意义。方法：取９３例肝癌患者、１５例肝炎后肝硬化患者、１２例慢性肝炎患者及１０例健康人静脉血各５ｍｌ，应用巢式ＲＴ－ＰＣＲ检测其周围静脉血中ＡＦＰｍＲＮＡ。结果：在５０个肝癌血标本中检测到ＡＦＰｍＲＮＡ（５３．８％），而对照组中未检测到１例。ＡＦＰｍＲＮＡ检出率与肝癌分期、血清ＡＦＰ浓度、肝内转移、门静脉癌栓、肿瘤大小及远处转移有明显相关（Ｐ＜０．０５）。２１例伴有肝外远处转移的原发性肝癌血标本均被检测到ＡＦＰｍＲＮＡ（１００％），没有远处转移的７２例肝癌血标本中有２９例检测到ＡＦＰｍＲＮＡ（４０．３％）。结论：ＡＦＰｍＲＮＡ可以作为肝癌细胞血液转移的标志。
Objective: To detect circulating hepatocellular carcinoma (HCC) cells by demonstrating HCC cells associated mRNA in the nuclear cell component of peripheral blood (PBL). Methods:Peripheral blood (5 ml) samples were obtained from 93 patients with HCC and from 37 control subjects (15 with liver cirrhosis after hepatitis B, 12 with chronic hepatitis B and 10 with normal liver function ). To identify HCC cells in PBL, liver specific human alpha fetoprotein ( AFP) mRNA was amplified from total RNA extracted from whole blood by reverse transcription polymerase chain reaction. Results: AFP mRNA was detected in 50 blood samples from the HCC patients (53.8%). In contrast, there were no clinical control patients whose samples showed detectable AFP mRNA in PBL. The presence of AFP mRNA in blood seemed to be correlated with the stage (by TNM classification ) of HCC, the serum AFP value, and the presence of intrahepatic metastasis, portal vein thrombosis, tumor diameter and /or distant metastasis ( P <0 05) . In addition, AFP mRNA was detected in the blood of 21 patients showing metastasis at extrahepatic organs (100%), in contrast to 29 of 72 cases without metastasis (40.3%). Conclusion: The presence of AFP mRNA in PBL may be an indicator of malignant hepatocytes, which might predict hematogenous spreading metastasis of tumor cells in patients with HCC.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第2期121-123,共3页
Academic Journal of Second Military Medical University
