摘要
目的:定量地寻找影响HPLC色谱参数的因素。方法:以泽泻HPLC指纹图谱的实验研究为模型,采用偏最小二乘法,结合均匀设计试验,研究确定最佳色谱条件的方法。结果:通过实验结合偏最小二乘法,得到了色谱参数与影响因素间的定量关系式,最终确定了泽泻的HPLC最佳色谱条件。结论:偏最小二乘法适于HPLC色谱等复杂系统的系统研究,可用于HPLC色谱条件的优化选择,实验次数少,时间短,结果准确度高,是一种科学、经济、快速的方法。
Objective： To quantificationally find the factors that affect the Chromatogratographic parameters . Method： In this paper, the HPLC fingerprint of Alisma Orientatis as a experimental model, using partial least squares method, combined with uniform design, to research how to determine the optimum chromatographic conditions. Results： In the experimental, combined with PLS , the relational expression of influence factor and chromatographic conditions was found out . Conclusion： PLS applies to the systematic studies in such complicated systems as chromatographic condi- tions of HPLG fingerprint, and it is a highly scientific, economic and rapid method.
出处
《内蒙古中医药》
2010年第11期149-150,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
基金
成都中医药大学校基金(ZRYB200509)
关键词
偏最小二乘法
优化选择
色谱条件
Partial Least Squares , Optimization, Chromatographic Conditions
