摘要
目的:通过动态检测大鼠肝癌模型造模各阶段血清中AFP-L3、HSP70和AFP水平的变化,对其在肝癌早期诊断中的价值做综合评价。方法:SD大鼠CCl4橄榄油溶液腹壁皮下注射,食用白酒溶液代替饮用水,建立大鼠肝硬化基础上的肝癌模型。从造模第6周起,每2周心脏采血1次,ELISA法检测大鼠血清中AFP-L3、HSP70和AFP水平,并取部分肝组织行病理学检验造模进度。结果:造模第8周发生肝硬化,第10周发生不典型增生及早期肝癌结节。AFP-L3和HSP70在造模第10周即显著升高,其阳性率分别为60%和70%,两者联合检测的阳性率为90%,HSP70在肝硬化阶段也有升高,AFP-L3没有显著变化。AFP水平在造模第12周才有显著升高,阳性率为60%,肝硬化阶段也略有升高,肝癌早期无显著变化。AFP-L3/AFP比值在造模第10周即显著升高(35.5%),其敏感性和特异性分别达70%和100%。结论:成功建立了大鼠肝硬化基础上的肝癌模型,传统指标AFP对极早期的肝癌不敏感,特异性不高,而AFP-L3和HSP70均较AFP敏感,两者联合可提高检测的敏感性,AFP-L3和AFP-L3/AFP比值均较AFP特异。
Objective：To evaluate the evaluation of these molecules in hepatoma early diagnosis by detecting AFP-L3,HSP70 and AFP level in different stages of the rat hepatoma model.Methods：Gave SD rat CCl4 oleum olivae solutions by subcutaneous injections,used edible distillate spirit to replace drinking water,so to establish rat hepatoma model based on liver cirrhosis.Drew blood from the heart every two weeks from the sixth week following the beginning of the model establishing,used ELISA to detect the AFP-L3,HSP70 and AFP level in the rat serum,and took part of the liver tissue to do pathology inspection to see the progression of the model establishing.Results：The cirrhosis appeared on the eighth week,haptoma appeared on the tenth week.AFP-L3 and HSP70 level stepped up significantly in the tenth week,the positive rates were 60% and 70% respectively,the positive rate of combined detection was 90%.HSP70 also stepped up in the stage of cirrhosis but AFP-L3 had no significant changes.AFP appeared later on the twelfth week,the positive rate was 60%.There was a little set-up in the stage of cirrhosis and had no significant difference between early haptoma.AFP-L3/AFP ratio set up significantly in the tenth week（35.5%）,the sensibility and specificity were 70% and 100% respectively.Conclusion：We have established the haptoma model based on cirrhosis successfully.The traditional index AFP is not very sensitive in the very early stage of hepatoma and also not very specific,but AFP-L3 and HSP70 are more sensitive than AFP in the early diagnosis of hepatoma,combined detection of AFP-L3 and HSP70 can raise the sensitivity,AFP-L3 and AFP-L3/AFP ratio are more specific than AFP.
出处
《中国医药导报》
CAS
2010年第22期13-15,18,共4页
China Medical Herald
基金
山东省潍坊医学院青年教师科研启动基金(KQ2007027)
