摘要
目的 观察晚期糖基化终末产物受体（RAGE）在不同程度急性肺损伤（ALI）大鼠血浆和肺泡灌洗液（BALF）中的变化,探讨RAGE在判断ALI严重程度方面的价值.方法 30只SD大鼠随机分为3组：低剂量组、高剂量组和对照组.Western blot法检测BALF和血浆中RAGE相对含量,同时测定PaO2、肺组织湿干质量比值（W/D）、BALF蛋白含量、血浆和BALF中肿瘤坏死因子-α（TNF-α）水平,结合病理改变评价各组肺损伤程度.结果致伤组均出现肺损伤,高剂量组损伤更明显;与对照组比较,两致伤组血浆和BALF中RAGE含量均有所上升（P﹤0.05）,且高剂量组BALF中RAGE含量又高于低剂量组（P〈0.05）,而血浆中RAGE含量在低剂量组与高剂量组间差异无统计学意义.结论 ALI时,BALF和血浆中RAGE水平升高,BALF中RAGE水平升高的程度可反应肺损伤的程度,RAGE可作为ALI/ARDS的预警指标.
Objective To investigate plasma and bronchoalveolar lavage fluid（BALF） level of receptor for advanced glycation end products （ RAGE ） in rat with different grade of experimental lung injury, exploring the value of RAGE in predicting the severity of ALI. Methods 30 Sprague - Dawley rats were randomly assigned into three groups（ n = 10） ： low dose group, high dose group and control group, Western blot was employed to detect the content of RAGE in plasma and BALF, the PaO2, wet/ dry weight（W/D） of lung lobes, content of protein level in BALF, TNF -α content in plasma and BALF were examined, the change of lung pathology were observed also. Results The Different degrees of ALI in rats were induced by each dose of OA ＋ LPS, higher dose group created more sever lung injury, the content of BALF and plasma RAGE increased in model groups than those of control group（P 〈 0. 05 ）, BAL RAGE in higher dose group elevated compared with that in other groups（P 〈 0.05 ）. Conclusion The level of RAGE in plasma and BALF increased in ALI, the increasing level of RAGE correlated with the severity of lung injury.
出处
《中国急救医学》
CAS
CSCD
北大核心
2010年第7期611-613,F0003,共4页
Chinese Journal of Critical Care Medicine
基金
基金项目：重庆市卫生局课题（No.2008-2-224）
