摘要
目的探讨清热燥湿方对脂多糖（LPS）诱导的急性肺损伤（ALI）大鼠支气管肺泡灌洗液（BALF）中中性粒细胞弹性蛋白酶（NE）和肺组织肿瘤坏死因子-a（TNF-a）蛋白及mRNA表达的影响。方法将40只雄性Wistar大鼠随机分为对照组、模型组、地塞米松组、清热燥湿方组，每组再分为4h和8h两个亚组，每个亚组5只。尾静脉注射LPS10mg／kg制备大鼠ALI模型。地塞米松组和清热澡湿方组分别于制模前灌胃地塞米松0．45mg／kg和清热燥湿方6．48g／kg。分别采用酶联免疫吸附法、免疫组化法及实时荧光定量聚合酶链反应（PCR）检测NE含量和TNF—a蛋白及mRNA的表达；同时进行肺组织病理观察。结果与模型组相比，清热燥湿方组4h、8hBALF中NE含量均显著降低（均P〈0．01）；地塞米松组和清热燥湿方组4h时TNF—a蛋白表达及4h、8h时TNF—amRNA表达均显著降低（P〈0．05或P〈0．01）。组织病理学观察显示，模型组大鼠肺组织出现大片出血及坏死；清热燥湿方组大鼠肺组织病理损伤程度较模型组减轻。结论清热燥湿方能减轻LPS致ALI大鼠肺组织损伤，其机制可能与清热燥湿方能降低LPS致ALI大鼠体内NE含量和TNF—a的表达有关。
Objective To investigate the effects of Qingre Zaoshi decoction （清热燥湿方） on neutrophil elastase （NE） in bronchial alveolar lavage fluid （BALF） and lung tissue expressions of tumor necrosis factor-a （TNF-a） protein and mRNA in the rats with acute lung injury （ALI） caused by lipopolysaccharide （LPS）. Methods Forty male Wistar rats were randomly divided into four groups., control, model, dexamethasone and Qingre Zaoshi decoction groups. Each group had two subgroups, 4 hours and 8 hours after LPS were injected. Each subgroup had 5 rats. The ALI rat model was established by intravenous injection of LPS （10 mg/kg） through a tail vein. The rats in dexamethasone group or Qingre Zaoshi decoction group were pretreated by gastric infusion of dexamethasone （0. 45 mg/kg） or Qingre Zaoshi decoction （6.48 g/kg） respectively for 3 days before ALI induced by LPS. Then the expression of NE was measured by enzymelabeled immunosorbent assay （ELISA）, the expressions of TNF-a protein and mRNA were measured by immunohistochemistry ABC and real-time fluorescent polymerase chain reaction （PCR）, while the histopathology of the lung injury was observed by light microscope. Results Compared with model group, the expression of NE in BALF in Qingre Zaoshi decoction group was obviously decreased at 4 hours and 8 hours （both P〈0.01）, and the expression of TNF-a protein positive area rate at 4 hours and TNF-a mRNA at 4 hours and 8 hours were obviously decreased in dexamethasone group and Qingre Zaoshi decoction group （P〈 0.05 or P〈 0.01）. Light microscope observation indicated that there were large areas of pulmonary hemorrhage and necrosis in model group, while in the Qingre Zaoshi decoction group, the pathological manifestations were much milder than those of the model group. Conclusion Qingre Zaoshi decoction ameliorates the injury of lung tissue in rats with ALI induced by LPS, thus it has protective effects on lung injury; the mechanism is possibly related to the inhibition of the expressions of NE in BALF and TNF-a protein and mRNA in injured lung tissue induced by LPS.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2010年第4期218-220,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
基金项目：上海市教委高校高水平特色发展项目[沪教委财（2005）81号]
上海市高等学校科学技术发展基金项目（04CB17）
上海市重点学科建设项目（S30301）
上海中医药大学名师研究室资助项目（2005-62）
