摘要
利用16SrDNA通用引物扩增了水稻褐飞虱Nilaparvata lugens(Stal)体内共生细菌的序列,经克隆、测序和NCBI数据库比对,发现褐飞虱体内存在杀雄菌属Arsenophonus类共生细菌,系统发育上与粉虱科和木虱科体内的Arsenophonus属亲源关系较近。在褐飞虱体内该共生细菌具有两种长度不同的16SrDNA序列,分别为1504bp和547bp,其中后者为前者中间缺失了957bp,其余序列相同。通过重新设计两对引物进行扩增,进一步确认不同褐飞虱地理种群及寄主种群均存在两种片段。Arsenophonus特异的23SrDNA引物的扩增结果表明,Arsenophonus存在于所有检测的褐飞虱种群中,但不存在于水稻寄主中。荧光定量PCR检测发现3个褐飞虱室内寄主种群Arsenophonus属共生细菌含量不同,其中TN1种群明显高于Mudgo种群和ASD7种群。此为水稻褐飞虱体内存在Arsenophonus属共生细菌的首次报道。
PCR amplification of 16S rDNA with universal primers was conducted to detect the symbiotic bacteria of the rice brown planthopper,Nilaparvata lugens （Stl）.Analysis of the sequences showed that the brown planthoppers contain the symbiotic bacteria Arsenophonus,which has close relationship with Arsenophonus symbiont of Psyllidae and Aleyrodidae based on phylogenetic analyses.Two 16S rDNA sequences of Arsenophonus in N.lugens with different sizes were amplified,one is 1 504 bp and the other is 547 bp in length.The shorter one is the deleted product of the longer one by loss of its internal region of 957 bp.With two primers redesigned based on the above 16S rDNA sequences,the result of PCR amplification showed that the two different fragments existed in various host-associated populations and different geographic populations of N.lugens.Using 23S rDNA specific primers,the genus Arsenophonus was found in all the tested populations of N.lugens,but not in the host rice plant.The TN1 population carried significantly higher amount of Arsenophonus bacteria than the Mudgo population and ASD7 population assessed by quantitative real-time PCR.This is the first report of Arsenophonus bacteria from N.lugens.
出处
《昆虫学报》
CAS
CSCD
北大核心
2010年第6期647-654,共8页
Acta Entomologica Sinica
基金
国家重点基础研究发展规划("973"计划)项目(2010CB126202)
公益性农业行业专项(200803003)
中央级公益性科研院所基金项目(2009RG004-3)
国家重点实验室自主研究课题(ZZKT200804)
关键词
褐飞虱
共生菌
杀雄菌属
16S
RDNA
系统发育
Nilaparvata lugens
symbiotic bacteria
Arsenophonus
16S rDNA
phylogenetic relationship
