摘要
目的:观察高脂饮食对大鼠血管内皮功能的影响并探讨其具体机制。方法:将30只8周龄雄性SD大鼠随机分为正常饮食组及高脂饮食组,喂养14周,麻醉后下腔静脉采血,测定甘油三酯、胆固醇、空腹血糖及胰岛素水平;分离主动脉,观察胸主动脉对内皮依赖性血管舒张剂乙酰胆碱(ACh)及内皮非依赖性血管舒张剂(SNAP)的舒张反应;检测血管一氧化氮(NO)、过氧化物的生成量及gp91phox的表达水平,NO合酶(NOS)的活性。结果:高脂饮食组大鼠血脂、空腹血糖和胰岛素水平显著升高,胸主动脉对ACh反应明显降低,血管组织gp91phox表达显著上调,过氧化物的生成及诱导型NOS的活性水平显著增加。结论:高脂血症大鼠存在内皮功能障碍,其机制可能与高血脂引起的氧化/硝基化作用密切相关。
AIM： To observe the effect of high-fat diet on the endothelial functions in rats.METHODS： Male SD rats（8 week old） were randomly divided into 2 groups to receive a regular or a high-fat diet,respectively.After 14 weeks,the animals were anesthetized and caval blood was collected to determine the lipid profile,fasting blood glucose and insulin levels.The aorta of the animals was isolated to observe the response of vasorelaxation to endothelium-dependent vasodilator acetylcholine（ACh） and endothelium-independent vasodilator SNAP（S-nitroso-N-acetyl penicillamine）.In addition,the production of nitric oxide（NO） and superoxide,the expression of gp91phox,and the activity of NO synthase（NOS） in the aortic tissues were measured.RESULTS： The lipid profile,the levels of fasting blood glucose and insulin were significantly increased in the plasma of rats fed with high-fat diet.A dose-dependent vasorelaxation to ACh was reduced,and the expression of gp91phox,the production of superoxide and the activity of iNOS were enhanced in the aortic tissues of the rats fed with high-fat diet.CONCLUSION： High-fat diet induces endothelial dysfunction by increasing the oxidative and nitrative stresses.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第8期1494-1497,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30700308)
