摘要
目的确定rDNA的内转录间隔区Ⅰ(ITS-1)是否可作为我国杜氏利什曼原虫(Leishmania donovani)分类的分子遗传标记。方法对我国不同疫区杜氏利什曼原虫分离株(L.d.SD1、L.d.SC7、L.d.XJ3、L.d.JS12)的ITS-1进行PCR扩增、克隆、测序,并分析它们的ITS-1序列多态性。结果 4个不同疫区的利什曼原虫分离株(L.d.SD1、L.d.SC7、L.d.XJ3、L.d.JS12)经PCR均扩增出一条约320bp的ITS-1片段,其序列大小分别为:317bp、323bp、317bp、316bp。序列分析表明:序列之间在多处位点发生了不同类型的突变,存在明显的序列多态性,其中伽师县突变的频率最为频繁,序列多态性最为显著。结论 ITS-1可以作为Leishmania donovani种内分类的分子遗传标记,有利于我国内脏利什慢原虫病的防治、诊断和治疗。
Objective To determine whether the first internal transcribed spacer （ ITS-1） of ribosomal DNA （ rDNA） could be used as a genetic marker of Leishmania donovani with different geographic origins in China.Methods The ITS-1 rDNA from L.d.SD1 isolate,L.d.SC7 isolate,L.d.XJ3 isolate,and L.d.JS12 isolate was amplified by PCR,cloned,sequenced,and then analyzed for sequence polymorphism.Results ITS-1 from L.d.SD1 isolate,L.d.SC7 isolate,L.d.XJ3 isolate,and L.d.JS12 isolate had a sequence size of 317 bp,323 bp,317 bp,and 316 bp,respectively.Different types of mutations occurred in the four sequences of ITS-1.Mutation of the ITS-1 from L.d.JS12 isolate in particular was the most frequent and its sequence polymorphism was the most significant.Conclusion ITS-1 can serve as a molecular genetic marker of Leishmania donovani with different geographic origins in China.
出处
《中国病原生物学杂志》
CSCD
2010年第7期520-523,共4页
Journal of Pathogen Biology
基金
中国医学科学院病原生物所资助项目
关键词
杜氏利什慢原虫
PCR
内转录间隔区Ⅰ
序列多态性分析
Leishmania donovani
PCR
first internal transcribed spacer （ ITS-1）
sequence polymorphism analysis
