摘要
目的探讨重组人腺病毒p53导入不同时限后联合放疗对A549肺腺癌细胞生长抑制、凋亡和p53蛋白表达的影响。方法将外源性p53基因导入人肺腺癌细胞株A549中,加药后即刻、3、6、24和48h联合射线照射(单次4Gy)。MTT检测细胞生长抑制,流式细胞仪检测细胞凋亡,Western blot检测野生型p53蛋白的表达情况。结果加药6、24和48h后放疗组与加药未放疗组、加药即刻放疗组和加药3h后放疗组比较,A549细胞生长抑制率、凋亡率及p53蛋白表达水平均显著增高(P<0.05)。结论为获得对肿瘤细胞的最大杀伤作用,可将放疗时间选定在导入重组人腺病毒p53后6～48h内。
Objective To explore the effects of different durations of recombinant human adenovirus p53 introduction combined with radiotherapy on cell growth inhibition,cell apoptosis,and p53 protein expression in lung adenocarcinoma cell line A549.Methods Wild-type p53 gene was injected into human lung adenocarcinoma cell line A549.A549 cells were exposed to radiation（4 Gy）immediately,3,6,24,and 48 hours after p53 gene introduction.The cell growth inhibition,cell apoptosis,and expression of p53 protein were determined by MTT assay,flow cytometry,and Western blot,respectively.Results Compared with A549 cells unexposed to radiation and those exposed to radiation immediately and 3 hours after p53 gene introduction,the inhibitory rate,apoptotic rate,and expression level of p53 protein were significantly higher in cells exposed to radiation 6,24,and 48 hours after p53 gene introduction（all P〈0.05）.Conclusion To gain the maximum cell-killing effect,the radiotherapy should be performed 6 to 48 hours after the introduction of adenovirus p53 gene.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第7期539-541,545,共4页
Journal of China Medical University
