摘要
目的了解宁波等7市野外家蝇自然种群对溴氰菊酯等6种杀虫剂的抗性动态,为科学合理使用杀虫剂,指导灭蝇工作的深入开展提供科学依据。方法采用点滴法,测定家蝇成蝇的半数致死剂量。结果经点滴法测定,宁波等7市家蝇自然种群对溴氰菊酯和高效氯氰菊酯的抗性为最高,抗性倍数依次为宁波市34.9、22.6倍,丽水市38.6、20.8倍,温州市36.4、23.5倍,衢州市28.3、30.5倍,金华市40.1、36.1倍,绍兴市34.1、32.0倍,临海市37.9、20.4倍;其次为敌敌畏和氯氰菊酯,抗性倍数依次为宁波市10.3、15.7倍,丽水市10.9、12.5倍,温州市11.2、17.5倍,衢州市13.8、12.6倍,金华市14.3、11.2倍,绍兴市10.7、16.3倍,临海市15.2、10.9倍;衢州、金华市对氯菊酯的抗性倍数分别为10.1和13.0倍,其他5个市对氯菊酯的抗性倍数均在8.2倍以下;而对三氯杀虫酯的抗性倍数均在1.9倍以下。宁波等7市家蝇自然种群与敏感品系比较,经方差分析两者之间差异有统计学意义(F=51.299,P=0.000);不同地区间自然种群的抗性比较差异无统计学意义(F=0.100,P=0.998);自然种群对不同杀虫剂间抗性发展水平表现不平衡,经方差分析差异有统计学意义(F=25.420,P=0.000)。结论自然种群对6种杀虫剂均产生了不同程度的抗性,以溴氰菊酯和高效氯氰菊酯最高。
Objective To determine the dynamics of the resistance of outdoor natural populations of Musca domestica to six insecticides including deltamethrin in seven cities such as Ningbo,providing the basis for scientific and proper use of pesticides and further development of fly control.Methods Topical application was used to determine the median lethal dose of the agents to M.domestica.Results The natural populations of M.domestica in the seven cities were most resistant to deltamethrin and beta cypermethrin,the resistance ratios for which were：34.9 and 22.6 times in Ningbo,38.6 and 20.8 times in Lishui,36.4 and 23.5 times in Wenzhou,28.3 and 30.5 times in Quzhou,40.1 and 36.1 times in Jinhua,34.1 and 32.0 in Shaoxing,and 37.9 and 20.4 times in Linhai.The resistance ratios for dichlorvos and cypermethrin,to which the flies were less resistant,were：10.3 and 15.7 times in Ningbo,10.9 and 12.5 times in Lishui,11.2 and 17.5 times in Wenzhou,13.8 and 12.6 times in Quzhou,14.3 and 11.2 times in Jinhua,10.7 and 16.3 times in Shaoxing,and 15.2 and 10.9 times in Linhai.The resistance ratios of the flies in Quzhou and Jinhua to permethrin were 10.1 and 13.0 times,respectively,while all of those in the other five cities were below 8.2 times.The resistance ratio of all the flies to while acetophenate was below 1.9 times.Analysis of variance showed significant difference in the resistance between the natural populations of M.domestica in the seven cities and the susceptible lines（F=51.299,P=0.000）,though no statistical difference was found in the resistance between the natural populations from different regions（F=0.100,P=0.998）.Analysis of variance showed statistical difference in the varied insecticidal resistance of the natural populations to different agents（F=25.420,P=0.000）.Conclusion The natural populations demonstrated varied resistance to the 6 pesticides,and were most resistant to deltamethrin and beta cypermethrin.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2010年第4期336-338,共3页
Chinese Journal of Vector Biology and Control
关键词
家蝇
抗药性
半数致死剂量
方差分析
Musca domestica
Insecticide resistance
Median lethal dose
Analysis of variance
