摘要
从新鲜牛奶中筛选高产γ-氨基丁酸(GABA)的乳酸菌株fmbl12-4,通过形态特征、生理生化特征和16SrDNA序列分析,鉴定菌株为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.lactis)。当fmbl12-4的湿菌体与100mmol/LL-谷氨酸钠溶液(L-MSG)按1:20(m/V)混合,于30℃、100r/min振荡反应24h,转化液中GABA浓度达到82.36mmol/L。在质量浓度为2.5g/100mL L-MSG的MRS培养基中培养6d,GABA质量浓度达到4.68g/L。
A lactic acid bacterial strain with high γ-aminobutyric acid（γ-GABA）-producing ability,named fmbl12-4,was isolated from fresh milk.Based on the studies of morphological,physiological and biochemical characteristics and 16S rDNA sequence and phylogenic analyses,fmbl12-4 was identified as Lactococcus lactis subsp.lactis.After 24 h shake（100 r/min） incubation of the wet mycelia of fmbl12-4 in a 20-fold volume of 100 mmol/L L-monosodium glutamate solution at 30 ℃,aγGABA content of 82.36 mmol/L was obtained.Incubation in a MRS medium containing 25 g/L L-MSG for 6 days gave 4.68 g/L γ-GABA content.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第15期198-202,共5页
Food Science
基金
国家"863"计划项目(2007AA10Z357)
关键词
Γ-氨基丁酸
乳酸菌
筛选
鉴定
γ-aminobutyric acid
lactic acid bacteria
isolation and screening
identification
