摘要
目的:体外评估7种乳杆菌的免疫作用,探讨小鼠离体脾脏淋巴细胞在筛选免疫活性乳酸菌中应用的可行性。方法:用MTT法观察不同种属、不同剂量的活性和热致死乳杆菌对小鼠脾脏淋巴细胞增殖能力的影响。结果:活性和热致死乳杆菌单独作用都能促进体外淋巴细胞增殖且均表现出剂量依赖关系,当活性和热致死乳杆菌剂量为107CFU/mL时(即细菌与细胞的比例为10:1)的效果最为明显(P<0.05),且活菌制剂的免疫调节作用优于热致死菌体(P<0.05),其中活性罗伊氏乳杆菌、约氏乳杆菌和发酵乳杆菌刺激淋巴细胞的增殖指数(PI值)明显较ConA诱导组高(P<0.05)。结论:正常离体小鼠脾脏淋巴细胞可用于体外初步筛选具有潜在免疫活性的乳杆菌,从而缩小用于动物实验的目标菌株的范围。
Objective：To evaluate the immunomodulation effect of seven Lactobacillus strains in vitro and to explore the application of mouse spleen lymphocytes in the selection of lactic acid bacteria with immunological activity.Methods：The effects of viable/heat-killed Lactobacillus strains belonging to different species at varying cell suspension concentrations on mouse lymphocyte proliferation were explored by MTT assay in vitro.Results：Either viable or heat-killed Lactobacilli alone could accelerate the proliferation of spleen cells in a dose-dependent manner.The most obvious immunomodulation effect was observed when vaiable/dead Lactobacillus strain suspension was at 107 CFU/mL（bacteria/cell ratio of 10：1）（P 0.05）.Meanwhile,the immunomodulation effect of living Lactobacillus strains was better than that of heat-killed Lactobacillus strains（P0.05）.The lymphocyte proliferation indexes（PI value） of Lactobacillus reuteri,Lactobacillus johnsoni and Lactobacillus fermentum were significantly higher than that of ConA-induced group（P0.05）.Conclusion：Spleen lymphocyte-based assay can be used as a useful primary indicator for the selection of candidate strains and an animal model for the evaluation of immunological activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第15期253-256,共4页
Food Science
基金
中国博士后科学基金面上资助项目(20090460872)
东北农业大学创新团队资助项目(CXT007-2-1)
关键词
乳杆菌
淋巴细胞增殖
筛选模型
免疫活性
MTT比色法
Lactobacillus
lymphocyte proliferation
screening model
immunological activity
MTT assay
