摘要
从非洲加纳籽中提取纯化5-羟基色氨酸,在单因素实验的基础上,选择具有显著影响的因素(乙醇浓度、料液比、提取时间)优化球面对称设计优化,得到最佳提取工艺为:80%乙醇以10:1(v/w)的料液比,在70℃下提取2 h,共提取2次,最佳纯化工艺为:提取液以等体积石油醚(60～90°)萃取后浓缩,结晶,结晶物以3%(w/w)活性炭脱色30 min,产品总收率达9.25%,含量超过98%,工艺先进独特,已成功应用于工业生产。
This paper deals with extraction and purification of 5-hydroxytryptophan（5-HTP） from Griffonia simplicifolia in African. Based on single-factor experiment,selected three independent variablels,namely：ethonal volum fraction,solvent-solid and extracting time were affecting factors in extraction.Spherical symmetric design was used for eptimizing the extraction process of 5-HTP.The results showed that the optimum extraction conditions for 5-HTP were as follows：the ethonal volum fraction 80%,ratio of liguid to solid10：1 （v/w）,extraction temperature 70℃,extraction time 2 h,total extraction 2 times.The optimum purification conditions for 5- HTP were as follows：extract the extraction liquid with identical volum.Petroleum benzene（60 -90°）,decolorize 30 min with the amount of activated carbon 3%（w/w）.the total yield of the products about 9.25%,the content above 98%.
出处
《计算机与应用化学》
CAS
CSCD
北大核心
2010年第7期933-936,共4页
Computers and Applied Chemistry
关键词
加纳籽
5-羟基色氨酸
吸附
结晶
球面对称设计
griffonia simplicifolia
5-hydroxytryptophan
absorbtion
crystal
spherical symmetric design
