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中国荷斯坦奶牛OPN基因内含子4多态性及其与产奶性状的相关分析 被引量：4

Correlation Analysis between Intron 4 SNP of OPN Gene and Milking Traits in Chinese Holstein
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摘要 研究采用PCR产物直接测序的方法,对278头中国荷斯坦奶牛的OPN基因内含子4进行单核苷酸多态性分析,并研究了不同基因型与产奶性状的相关关系。结果表明:OPN基因内含子4有TT、CT和CC 3种基因型,基因型频率分别为0.3309、0.5108和0.1583;该位点突变处于Hardy-Weinberg平衡状态,且PIC为0.3676;CC基因型与中国荷斯坦奶牛的乳脂率和乳蛋白率呈显著相关。说明C等位基因对泌乳性状具有加性效应,OPN基因可以作为中国荷斯坦奶牛泌乳性状的候选基因。 The article studied single nucleotide polymorphism（SNP） of Osteopontin in 278 Chinese Holstein cattle by DNA sequencing.We investigated the correlation between genotype and milk production traits.Results showed TT,CT,CC existed in Chinese Holstein cattle and genotype frequency were 0.3309,0.5108,0.1583,respectively;the variable sites was in Hardy-Weinberg equilibrium and PIC was 0.3676;CC genotype had significant correlation with milk fat and protein percentage.Therefore,C allele had the additive effect and OPN gene could be regarded as a candidate gene effecting milk traits.
作者 王杰 原清会 曾长军 张明 赖松家
机构地区 四川农业大学动物科技学院
出处 《中国畜牧杂志》 CAS 北大核心 2010年第15期5-8,共4页 Chinese Journal of Animal Science
基金 四川农业大学博士后基金(04310605) 双支计划项目(01570402)
关键词 OSTEOPONTIN 单核苷酸多态性 产奶量 乳脂率 乳蛋白率 中国荷斯坦奶牛 osteopontin SNP milk yield fat percentage protein percentage Chinese Holstein
分类号 S823.2 [农业科学—畜牧学]
  • 相关文献

参考文献10

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二级参考文献18

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共引文献4

同被引文献37

  • 1何云刚,金力,黄薇.单核苷酸多态性与连锁不平衡研究进展[J].基础医学与临床,2004,24(5):487-490. 被引量：16
  • 2唐剑频,侯一平.基质辅助激光解吸/离子化飞行时间质谱分析多态性遗传标记[J].中华医学遗传学杂志,2005,22(2):185-188. 被引量：10
  • 3赵辉,王威,张清润,高扬,赵洪斌,周珺,林伟,曾长青.高通量飞行时间质谱基因分型方法的研究[J].生物化学与生物物理进展,2005,32(7):667-672. 被引量：20
  • 4何峰,孙东晓,俞英,王雅春,张沅.荷斯坦奶牛STAT5A基因的SNPs检测及其与产奶性状的关联分析[J].畜牧兽医学报,2007,38(4):326-331. 被引量：16
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  • 8Inman C K and Shore P, 2003. The osteoblast transcription factor Run-2 is expressed in mammary epithelial cells and mediates osteopontin expression. The Journal of Biological Chemistry. 278(49): 48684-48689.
  • 9Khatib H, Zaitoun I, Wiebelhaus-Finger J, Chang Y M and Rosa G J M, 2007. The association of bovine PPARGCIAand OPN genes with milk composition in two independent holstein cattle populations. Journal of Dairy Science, 90: 2966-2970.
  • 10Leonard S, Khatib H, Schutzkus V, Chang Y M and Maltecca C, 2005. Effects of the osteopontin gene variants on milk production traits in dairy cattle. Journal of Dairy Science, 88:4083-4086.

引证文献4

二级引证文献14

中国畜牧杂志
2010年 第15期

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