摘要
目的:研究中药夏枯草对人膀胱癌细胞系T24细胞FasLmRNA表达及对其侵袭能力的影响。方法:根据MTT法得到中药夏枯草对T24细胞的半数有效抑制浓度(IC50),确定药物作用浓度。T24细胞分别经中药夏枯草不同浓度(0.5IC50、IC50)作用后,应用逆转录-聚合酶链反应(RT-PCR)法检测中药夏枯草作用前后人膀胱癌细胞系T24细胞FasLmRNA的变化;应用Transwell细胞侵袭试验检测中药夏枯草对T24细胞侵袭能力的影响。结果:中药夏枯草处理后较处理前T24细胞FasLmRNA表达水平均明显高于对照组(P<0.05);而且FasLmRNA表达水平随中药夏枯草作用浓度增加显著上调,中药夏枯草不同浓度组比较均有显著性差异(P<0.05);随中药夏枯草作用浓度升高,T24细胞侵袭能力明显增强,不同浓度组比较均有显著性差异(P<0.05)。结论:中药夏枯草在一定时间内均可上调人膀胱癌细胞系T24细胞FasLmRNA的表达,而且这种上调作用在一定范围内呈剂量依赖性,可使膀胱癌细胞的侵袭能力增强。
Objective：To investigate the effect of traditional Chinese medicine Prunella vulgaris on Fas Ligand mRNA expression of human Bladder Cancer Cell line T24.Methods：The inhibition concentration 50% （IC50） of Prunella vulgaris was detected by MTT assay.Bladder Cancer Cell line T24 were treated by different concentrations （0.5IC50, IC50） of Prunella vulgaris.The changes of Fas Ligand mRNA were detected by the reverse transcription polymerase chain reaction method （RT-PCR） and invasive ability of T24 cells was measured by Transwell-ECM.Results：Fas Ligand mRNA increased after treated by Prunella vulgaris upregulated （p 0.05）; Transwell-ECM showed that the higher concentration Prunella vulgaris increased apoptosis rate of lymphocyte induced of T24 cells（p 0.05）.The different concentrations teams of Prunella vulgaris have statistical significance （p0.05）.Conclusion：Prunella vulgaris can up-regulated the expression of functional Fas Ligand mRNA of human Bladder Cancer Cell line T24.And, the up regulative action raised invasive ability ofcancer cells.
出处
《现代生物医学进展》
CAS
2010年第14期2628-2631,共4页
Progress in Modern Biomedicine
关键词
中药夏枯草
膀胱癌
侵袭
gene expression
Prunella vulgaris
Bladder Cancer Cell line T24
invasion
