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JNK信号通路参与Aβ毒性对大鼠海马神经元的损伤 被引量:5

JNK signal transduction pathway participated Aβ-induced neuronal injury in rat hippocampus
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摘要 目的探讨β-淀粉样蛋白(β-amyloid peptide,Aβ)毒性对大鼠海马神经元的损伤以及JNK信号转导通路在此过程中的作用。方法应用Aβ1-40注射大鼠双侧海马CA1区建立毒性损伤模型,通过HE染色、Nissl染色、TUNEL染色、免疫组化法、透射电镜并结合图像分析技术研究海马的病理学变化以及神经元的存活与凋亡、超微结构及p-JNK阳性细胞率。结果与对照组比较,大鼠注射Aβ后,海马CA1区锥体细胞排列松散,数目减少,神经元固缩、浓染,胶质细胞明显增生,超微结构可见明显凋亡特征,TUNEL阳性细胞数明显增多(P<0.01),p-JNK阳性细胞比例明显升高(P<0.01)。结论 Aβ可明显诱导海马神经元凋亡,JNK信号转导通路参与了Aβ的神经毒性损伤作用。 Aim To investigate the hippocampal neuronal injury induced by β-amyloid peptide ( Aβ) in rats and the candidate contribution of JNK signal transduction pathway to Aβ toxicity. Methods Rats were bilaterally injected with Aβ1 -40 into hippocampi CA1 area. The pathological changes,survival and apoptosis,and ultrastructure of hippocampal neurons,as well as p-JNK positive cells were observed by HE staining,Nissl staining,TUNEL staining,immunohistochemistry and transmission electron microscopy,respectively. Results Thepyramidal cells in hippocampus arranged loosely with decreased cell number. The gliacytes significantly proliferated. Significant apoptotic features were observed in CA1 ultrastructure. TUNEL-positive cells and p-JNK-positive cells significantly increased ( P〈0. 01) . Conclusions Aβ can significantly induce neuronal apoptosis in hippocampus in rats. JNK signal transduction pathway participates Aβ neuronal toxicity of apoptotic injury.
出处 《中国药理学通报》 CAS CSCD 北大核心 2010年第8期1034-1037,共4页 Chinese Pharmacological Bulletin
基金 国家自然科学基金资助项目(No30701102 30830188)
关键词 JNK信号转导通路 Β-淀粉样蛋白 海马 神经元 凋亡 大鼠 JNK signal transduction pathway β-amyloid peptide hippocampus neuron apoptosis rat
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参考文献5

  • 1Troy C M,Rabacchi S A,Xu Z,et al.β-amyloid-induced neuronal apoptosis requires c-Jun N-terminal kinase activation[J].J Neurochem,2001,77(1):157-64.
  • 2Lin A,Dibling B.The true face of JNK activation in apoptosis[J].Aging cell,2002,1(2):112-6.
  • 3丛伟红,刘建勋,徐立.人参、银杏叶提取物对β-淀粉样蛋白_(1-40)毒性损伤大鼠全脑神经递质的影响[J].中国药理学通报,2006,22(6):747-750. 被引量:13
  • 4Standridge J B.Vicious cycles within the neuropathophysiologic mechanisms of Alzheimer's disease[J].Curr Alzheimer Res,2006,3(2):95-108.
  • 5Li L M,Liu Q H,Qiao J T,Zhang C.Abeta(31-35)-induced neuronal apoptosis is mediated by JNK-dependent extrinsic apoptosis pathway[J].Neurosci Bull,2009,25(6):361-6.

二级参考文献16

  • 1赵伟康,金国琴,谢积荣,黄新生.固真方对老年大鼠海马和下丘脑单胺类神经递质含量的影响[J].中国老年学杂志,1994,14(6):368-369. 被引量:13
  • 2孟宪丽,曾南,张艺,赖先荣,任长茂,程龙琼.淫羊藿有效成分对老龄雄性大鼠下丘脑单胺类神经递质及其他脑功能作用的研究[J].中国中药杂志,1996,21(11):683-685. 被引量:56
  • 3Jung S S,Nalbantoglu J,Cashman N R.Alzheimers beta-amyloid precursor protein is expressed on the surface of immediately exvivo brain cell:a flow cytometric study[J].J Neurosci Res,1996,46(3):336.
  • 4刘建勋 丛伟红 徐立 王建农.改进的高效液相电化学法测定人参、银杏叶提取物配伍对β—淀粉样蛋白损伤大鼠脑内乙酰胆碱含量的影响[J].中国药理学报,2004,25(9):1118-23.
  • 5Auld D S,Kar S,Quirion R.Beta-amyloid peptides as direct cholinergic neuromodulators:A missing link[J].Trends Neurosci,1998,21(1):43-9.
  • 6Geula C,Wu C K,Saroff D M,et al.Aging renders the brain vulnerable to amyloid beta-protein neurotoxicity[J].Nat Med,1998,4(7):827-31.
  • 7Collerton D.Cholinergic function and intellectual decline in Alzheimers disease[J].Neuroscience,1986,19(1):1-28.
  • 8Sramek J J,Anand R,Wardle T S,et al.Safety/tolerability trial of SDZ ENA 713 in patients with probable Alzheimers disease[J].Life Sci,1996,58(15):1201-7.
  • 9Rogers S L,Farlow M R,Doody R S,et al.A 24-week,double-blind,placebo-controlled trial of donepezil in patients with Alzheimers disease[J].Neurology,1998,50(1):136-45.
  • 10Burke W J,Li S W,Schmitt C A,et al.Accumulation of 3,4-dihydroxyphenylglycolaldehyde,the neurotoxic monoamine oxidase A metabolite of norepinephrine,inlocusceruleuscellbodies in Alzheimers disease:mechanism of neuron death[J].Brain Res,1999,816(2):633-9.

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