摘要
研究了暴露于微小亚历山大藻藻液中的黑鲷仔鱼其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化物酶(CAT)和Na+-K+-ATPase酶活性在4d时间内的变化,结果表明,微小亚历山大藻藻液对仔鱼的GSH-PX和CAT活性诱导作用明显,尤其对处于染毒时间2d内,这种作用较强,但随着实验时间的延长又表现出抑制作用。微小亚历山大藻藻液对SOD也有强烈的诱导作用,这种作用可持续3d,但随后则表现为抑制作用。仔鱼的3种酶活性与染毒的藻液均符合较明显的剂量-效应关系,即实验藻液浓度越大,对酶活性的抑制率越低,对生物毒性越大。但藻液对于Na+-K+-ATPase酶却表现出强烈的抑制作用,显示出浓度-效应关系。其与上述3种酶的活力变化可以一起作为水体中藻液污染的生物指示器。
In this article, we studied the change of superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）, peroxidase （CAT） and Na＋-K＋-ATPase activity of larvae of Sparus macrocephalus exposed to Alexandrium minitum algae liquid. It revealed that Alexandrium minitum algae liquid had obvious induction to the GSH-PX and CAT of larvae of Sparus macrocephalus in the early exposure stage, especially for the first two days. However, it showed inhibition effect in the following experiment after 2 days. Besides, Alexandrium minitum algae liquid showed strong induction effect to SOD for the first 3 days then changed to the inhibition effect. The obvious dose-response link between the concentration of exposed algae solution and the activity of SOD, GSH-PX and CAT showed that the higher the concentration of algae solution, the lower the inhibition and the greater the biological toxicity. However, the strong inhibition of algae solution to Na^＋-K^＋-ATPase displayed concentration-response link. The four enzyme’s activity changes of the larvae of Sparus macrocephalus studied in this paper can work as the biological indicator of algae pollution in water.
出处
《海洋通报》
CAS
CSCD
北大核心
2010年第4期427-431,共5页
Marine Science Bulletin
基金
国家重点基础研究发展规划"973"项目资助(G2010CB428705)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)(2007M08)
上海市科委"登山行动计划"项目(06dz2015)
