摘要
目的:观察UC大鼠结肠损伤与黏蛋白分泌情况,探讨不同灸法对UC大鼠结肠损伤的修复作用。方法:60只SD大鼠随机数字表法分为正常组、模型组、隔药灸组、隔姜灸组、隔蒜灸组和温和灸组6组。采用免疫学方法,并加局部刺激制备实验性溃疡性结肠炎大鼠模型。隔药灸组、隔姜灸组、隔蒜灸组和温和灸组取天枢穴(双)、气海穴,分别施以相应的治疗,连续治疗14次。疗程结束后,分别观察各组动物的体重、大便、结肠大体形态及组织病理学改变、结肠黏膜黏蛋白分泌情况。结果:溃疡性结肠炎大鼠体重增长缓慢,出现腹泻症状;病理学观察可见溃疡形成;结肠黏膜中性黏蛋白和酸性黏蛋白分泌减少,与正常组比较具有显著差异(P<0.01)。隔药灸组、隔姜灸组、隔蒜灸组、温和灸组经相应治疗后,大鼠体重增加,腹泻症状得到明显改善,结肠肠壁各层结构明显改善。除温和灸组溃疡面尚未愈合外,隔药灸组、隔姜灸组、隔蒜灸组溃疡面已经基本愈合。结肠黏膜中性黏蛋白和酸性黏蛋白分泌上调,与模型组比较具有显著差异(P<0.05~0.01)。结论:隔药灸、隔姜灸、隔蒜灸、温和灸可以通过调节黏蛋白表达,起到修复UC大鼠结肠损伤的作用。
Objective:To investigate the injury of colon and the secretion of mucin in rats of ulcerative colitis treated by different moxibustion. Methods:60 rats were randomly assigned to normal,model,herb-partition moxibstion,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion groups. A rat model of UC was established by an immunological method plus local stimulation. Points bilateral Tianshu and Qihai were selected for herb-partition moxibstion,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion groups,the treatment continued for 14 times. After treatment,the weight,stool,general form and morphology changes of colonic tissues were observed. The secretion of mucin was examined. Results:The weight of model rats increased slowly,and the diarrhea sign was occur,the ulcer were observed in pathology;the neutral and acor mucin excretion were significantly reduced compared with normal group (P〈0.01).The weight,stool system and colon structure of rats were improver by the treatment of herb-partition moxibstion ,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion.Apart of the warm moxibustion group,the ulcer almost have been healed in herb-partition moxibstion group,ginger-partition moxibustion group,garlic-partition moxibustion group. The excretion of neutral and acor mucin were up-regulated compared with model group in colon mucosa (P〈0.05-0.01). Conclusion:The injured colon mucosa can be recoved by herb-partition moxibstion ,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion,through by regulating the mucin expression.
出处
《中华中医药学刊》
CAS
2010年第8期1590-1594,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30200368)
国家重点基础研究发展计划(973计划)资助项目(2009CB522900)