摘要
利用PCR扩增人源TPA基因,再用酶切-连接的方法将目的片段亚克隆入慢病毒表达质粒pL-PGK-eGFP中,最后用测序、酶切和在293细胞中瞬时表达的方法进行了鉴定.结果显示:含人源TPA基因的pL-PGK-TPA-eGFP慢病毒表达载体构建成功,为进一步利用转基因技术更加安全高效地生产TPA,治疗血栓类疾病打下基础.
The gene TPA was amplified by PCR,then the fragment was subcloned into the lentiviral vector pL-PGK-eGFP,and was confirmed by EheI digestion,sequencing and instantaneous expressing.The results of research showed that a lentiviral vector pL-PGK-TPA-eGFP containing human TPA gene was constructed.It will provide foundation for the production of TPA by transgene technology to treat thrombus.
出处
《安徽师范大学学报（自然科学版）》
CAS
北大核心
2010年第3期267-271,共5页
Journal of Anhui Normal University(Natural Science)
基金
"十一五"国家科技支撑计划项目(No2006BAD04A01
2006BAD04A12)
芜湖市科技计划重点项目(2008620)
安徽省自然科学基金项目(050410201)
