摘要
目的：建立胃安胶囊中盐酸小檗碱的含量测定方法。方法：采用HPLC法测定。SHMADZUVP—ODS（250mm×4．6mm，5μm）为色谱柱；乙腈-四氢呋喃-0．1％磷酸溶液（46：2：52）为流动相；流速为1．0ml／min；检测波长265nm；柱温30℃。结果：盐酸小檗碱在1．02-102．2μg／ml的范围内具有良好的线性关系（r=0．9998），平均回收率为99．1％，RSD为0．50％（n=9）。结论：该方法简便、准确、重现性好，适用于胃安胶囊中盐酸小檗碱的含量测定和质量控制。
Objective： To establish a HPLC method for determination of berberine hydrochloride in Weian capsule.Methods： Column was VP-ODS（250 mm×4.6 mm,5 μm）;mobile phase was acetonitri-tetrahydrofuran-waters with 0.5% phosphoric acid（46∶2∶52）;the flow rate was at 1.0 ml/min;the column temperature was 30 ℃;the detection wavelength was 265 nm.Results： The linear range of betaine hydrochloride was 1.02~102.2 μg/m（r=0.9998）.The average recovery（n=9） was 99.1%,with RSD 0.5%.Conclusion： The method is available and accurate,and can be used to the determination and quality control of berberine hydrochloride contents in Weian capsule.
出处
《华夏医学》
CAS
2010年第2期154-157,共4页
Acta Medicinae Sinica
