摘要
目的观察烫伤后重组杀菌通透性增加蛋白（ｒＢＰＩ２１）对内毒素增敏系统脂多糖结合蛋白／脂多糖受体ＣＤ１４（ＬＢＰ／ＣＤ１４）及肿瘤坏死因子α（ＴＮＦα）ｍＲＮＡ表达的影响及其意义。方法采用大鼠３５％Ⅲ°烫伤模型，动物分为正常对照组（８只）、烫伤组（１２只）和ｒＢＰＩ２１治疗组（１２只）。烫伤组和ｒＢＰＩ２１治疗组动物分别于伤后１２和２４小时活杀。留取组织和血标本采用半定量ＲＴＰＣＲ方法，分别检测组织ＬＢＰ、ＣＤ１４、ＴＮＦαｍＲＮＡ表达及器官功能指标。结果ｒＢＰＩ２１治疗可明显抑制局部组织ＬＢＰ／ＣＤ１４ｍＲＮＡ表达（Ｐ＜００５００１），并不同程度抑制肝、肺及肾组织ＴＮＦαｍＲＮＡ表达水平（Ｐ＜００５００１）。同时，ｒＢＰＩ２１治疗组动物血清谷丙转氨酶水平明显降低（Ｐ＜００１），而小肠组织二氨氧化酶活性显著高于烫伤组（Ｐ＜００５）。结论烫伤早期应用ｒＢＰＩ２１可部分减轻肠源性内毒素移位诱发的病理损害，其作用机理可能与ｒＢＰＩ２１对体内多种组织ＬＢＰ／ＣＤ１４ｍＲＮＡ表达的下调效应有关。
Objective To elucidate the possible mechanisms underlying beneficial effect of recombinant bactericidal/permeability increasing protein (rBPI) on multiple organ damage secondary to major burns. Methods Wistar rats were subjected to a 35% full thickness scald injury, and randomly divided into 3 groups:normal controls (n=8), thermal injury (n=21), and rBPI 21 treatment (n=12). In thermal injury and rBPI 21 treatment groups, animals were sacrificed at 12 and 24 hours after burn, respectively. Tissue samples from liver, kidneys, lungs and intestine were collected to measure lipopolysaccharide binding protein (LBP)/lipopolysaccharide receptor CD14 and tumor necrosis factor α (TNF α) mRNA expression. In addition, blood samples were obtained for measurement of organ function parameters. Results Treatment with rBPI 21 could significantly decrease tissue LBP and CD14 mRNA expression in various organs ( P <0 05 0 01). Also, TNF α mRNA expression in liver, kidneys and lungs were markedly inhibited by rBPI 21 secondary to acute insults ( P <0 05 0 01). Moreover, in the rBPI 21 treatment group, significant reduction in serum GPT levels and elevation in intestinal DAO activities were found compared to those in the thermal injury group ( P <0 05 0 01). Conclusions Treatment with rBPI 21 is effective in attenuating multiple organ damage resulted from gut origin endotoxin translocation after thermal injury, which might be associated with down regulation effect of tissue LBP and CD14 gene expression by use of rBPI 21 .
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第4期289-291,共3页
National Medical Journal of China
基金
全军九五医药卫生科研基金
中国工程科技奖励基金
关键词
烫伤
脂多糖
rBPI21
CD14抗原
mRNA
TNF
Burns
Lipopolysaccharide binding protein
CD14
Tumor necrosis factor α
Recombinant proteins
