摘要
目的：建立五淋散胶囊中栀子苷和芍药苷的HPLC含量测定方法。方法：采用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）,流动相为甲醇-水（20∶80）,流速为1.0 mL.min-1,检测波长为235 nm;柱温为30℃;进样量为20μL。结果：栀子苷进样量在0.162-1.296μg范围内线性关系良好（r=0.999 9）,平均回收率为101.30%,RSD为0.54%（n=6）;芍药苷在0.160-1.280μg范围内线性关系良好（r=0.999 9）,平均回收率为99.75%,RSD为1.04%（n=6）。结论：本方法简便、准确、快速、灵敏度高、重现性好,可作为五淋散胶囊中栀子苷和芍药苷的定量控制质量标准。
Objective：To establish a HPLC method for the determination of geniposide and paeoniflorin in Wulinsan capsule.Methods：The separation was performed on a Diamonsil C18 chromatographic column（250 mm×4.6 mm,5 μm）with the mobile phase of methyl alcohol-water（20∶80） at the flow rate of 1.0 mL·min^-1.UV detection wave-length was at 235 nm.Column temperature was at 30 ℃.Results：The geniposide showed a good linearity in the range of 0.162-1.296 μg（r=0.999 9）.The average recovery was 101.30%,and RSD was 0.54%（n=6）.The paeoniflorin showed a good linearity in the range of 0.160~1.280 μg（r=0.999 9）.The average recovery was 99.75%,and RSD was 1.04%（n=6）.Conclusion：This method is simple,accurate,sensitive and reproducible,then it can be used for controlling the quantitative of geniposide and paeoniflorin in Wulinsan capsule.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第14期1281-1283,1288,共4页
Chinese Journal of New Drugs
基金
广西南宁市科技攻关项目(2008-2095C)
