摘要
目的探讨Wistar大鼠脑震荡模型是否可以做为脑震荡伴有迷路震荡的模型。方法 40只健康雄性Wistar大鼠随机分成4组,分别为A组(正常对照),B组(撞击后30min),C组(撞击后1h)以及D组(撞击后1d),各组均为10只20耳。选用0.5、0.7、1.0、1.4、2.0、3.0、4.0、6.0和8.0kHz共9个频率点进行畸变产物耳声发射(distortion product oto-acoustic emissions,DPOAE)(f_2/f_1=1.2,L_1=65dBSPL,L_2=55dBSPL)幅值测试,同时进行脑干诱发电位(auditory branistem response,ABR)阈值检测。并将大鼠处死后扫描电镜观察耳蜗毛细胞的变化。结果撞击后B、C、D组与A组相比,ABR反应阈值升高,DPOAE在2.0kHz时幅值明显下降,差异有统计学意义(P<0.05)。扫描电镜观察耳蜗中转和底转在撞击后B组外毛细胞排列不整齐,D组有轻微倒伏现象。结论 Wistar大鼠脑震荡模型可以做为脑震荡伴有迷路震荡的模型。
Objective To study if the Wistar rat model of cerebral concussion may be developed as the model of labyrinthine concussion. Methods Forty healthy male Wistar rats were divided into group A （ the control group）, B （30 minutes after impact） , C （ 1 hour after impact） and D （ 1 day after impact）. The distortion product oto - acoustic emissions （DPOAE） audiogram （ f2/fl = 1. 2, L1 = 65dBSPL, L2 = 55dBSPL） at 0.5,0.7,1.0,1.4,2.0,3.0,4.0,6.0 and 8.0 kHz were analyzed in the three circumstances. The values of auditory branistem response （ABR）were simultaneously measured. The changes of cochlear hair cells were observed under the scanning electron microscope. Results The increased values of ABR were observed in group B, C and D （ P 〈 0.05 ） . The DPOAE significantly decreased at 2.0 kHz after rats were rammed in group C and group D （ P 〈 0.05）. cochlea outer hair cells on the intermediate and basefond cireumvolutio showed disordered in group B and lightly lodging in the group D by scanning electron microscope. Conclusion The animal model of cerebral concussion can be used as the model of labyrinthine concussion in Wistar rats.
出处
《河北医科大学学报》
CAS
2010年第7期799-801,F0003,共4页
Journal of Hebei Medical University
基金
河北省科技支撑计划项目(08276101D-35)
