摘要
目的探讨血浆与脑脊液（ＣＳＦ）中内皮素（ＥＴ）、一氧化氮（ＮＯ）在新生儿缺氧缺血性脑病（ＨＩＥ）中的作用及其相互关系。方法对３５例ＨＩＥ患儿（轻度１０例，中度１３例，重度１２例）分别采用放射免疫法测定血浆和ＣＳＦ中ＥＴ１，硝酸根还原酶法测定血浆和ＣＳＦ中ＮＯ－２／ＮＯ－３值代表ＮＯ水平，并观察其急性期、恢复期的动态变化。设１５例作为对照组。结果急性期ＨＩＥ患儿血浆和ＣＳＦ中ＥＴ１水平中、重度组明显高于对照组，尤以重度组升高显著，轻度组与对照组差异无显著意义；恢复期轻、中度组ＥＴ１水平降至正常，但重度组仍维持在较高水平，与对照组比较，差异有显著意义。急性期ＨＩＥ患儿血浆和ＣＳＦ中ＮＯ水平除轻度组外，中、重度组明显高于对照组，重度组又明显高于轻度组；恢复期轻、中、重度组血浆和ＣＳＦ中ＮＯ水平均降至正常，与对照组比较，差异无显著意义。结论ＨＩＥ患儿脑损伤与ＥＴ１、ＮＯ过量合成、功能失衡有关，血浆与ＣＳＦ中ＥＴ１持续不降者提示病情严重，预后不良。
Objective To study the relationship and the effects of endothelin1 (ET1) and nitric oxide (NO) in
plasma and cerebrospinal fluid (CSF) on hypoxic ischemic encephalopathy (HIE) Methods In 35
newborn infants with HIE (10 cases of mild, 13 cases of moderate and 12 cases of severe
status), ET1 levels in plasma and CSF were measured by radio immunoassay both in acute and
recovery phases; NO2/NO3 level of plasma and CSF were measured with nitrate reductase
Results In acute phase, the ET1 levels of plasma and CSF in infants with moderate and severe
HIE were much higher than those in controls, especially the severe cases There was no
significant difference of ET1 levels between the mild HIE and the control infants However, in
recovery phase, the ET1 in severe HIE infants kept at the high level In acute phase the NO
levels in plasma and CSF of the moderate and severe HIE infants were much higher than those
of the controls The NO levels of the severe HIE infants were obviously higher than those of the
mild ones In recovery phase, the NO levels in all three groups of the HIE infants decreased to
the normal levels, which were no significant differences in compared with the control
Conclusion The brain damage of the HIE infants might relate to the over expression of ET1 and
NOThe persistent increase of ET1 in plasma and CSF might be an indicator of a serious illness
and a poor prognosis
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1999年第6期362-364,共3页
Chinese Journal of Pediatrics
基金
浙江省教委科研基金
关键词
内皮素
一氧化氮
新生儿
缺氧缺血性脑病
Infant, newbornCerebral anoxiaCerebral
ischemiaEndothelinsNitric oxide
